Institutional Repository

Show simple item record

dc.contributor.advisor Alkadhi, Karim A.
dc.creator Alhaider, Ibrahim
dc.date.accessioned 2012-09-27T15:54:56Z
dc.date.available 2012-09-27T15:54:56Z
dc.date.created 2010-05
dc.date.issued 2012-09-27
dc.date.submitted May 2010
dc.identifier.uri http://hdl.handle.net/10657/ETD-UH-2010-05-31
dc.description.abstract Study objectives: Accumulating evidence has shown that caffeine and sleep deprivation have opposing effects on learning and memory; therefore, this study was undertaken to provide a detailed account of the effect of chronic, low-dose caffeine treatment on the deleterious effects of sleep loss on hippocampus-dependent learning and memory. Experimental design: We investigated the effects of chronic (4 weeks) caffeine treatment (0.3 g/l in drinking water) on memory impairment in acutely (24 hr) sleep-deprived rats. Sleep deprivation was induced using the modified multiple platform model. The effects of caffeine on sleep deprivation-induced hippocampus-dependent learning and memory deficits were studied using three approaches: learning and memory performance in the radial arm water maze task; electrophysiological recordings in the Cornu Ammonis (CA1) and dentate gyrus (DG) regions of the hippocampus; and western blot analysis to measure the levels of memory- and synaptic plasticity-related signaling molecules. Results: Our results showed that chronic caffeine treatment prevented impairment of hippocampus-dependent learning, short-term memory and early phase- long-term potentiation (E-LTP) of the CA1 and DG areas in the sleep-deprived rats. In correlation, caffeine treatment prevented a sleep deprivation-associated decrease in the basal levels of phosphorylated calcium/calmodulin-dependent protein kinase II (P-CaMKII) and brain-derived neurotrophic factor (BDNF). In addition, caffeine treatment of sleep-deprived rats increased the levels of P-CaMKII during the expression of E-LTP. The results also showed that chronic caffeine treatment prevented the impairment of long-term memory and late phase-LTP (L-LTP) in the CA1 and DG regions of the sleep-deprived rats. Additionally, caffeine treatment prevented a sleep deprivation-associated decrease in the basal levels of the phosphorylated cAMP response element binding protein (P-CREB) as well as total CREB. Treating sleep-deprived rats chronically with caffeine enables multiple high frequency stimulation to increase the levels of P-CREB during L-LTP expression. Conclusions: The results suggest that long-term use of a low dose of caffeine protects against the harmful changes in the basal levels of P-CaMKII, P-CREB and BDNF associated with sleep deprivation and as a result contributes to the revival of hippocampus-dependent learning and memory as well as LTP in the CA1 and DG regions.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subject caffeine
dc.subject sleep deprivation
dc.subject memory
dc.subject hippocampus
dc.subject long-term potentiation
dc.subject spatial memory
dc.subject CREB
dc.subject CaMKII
dc.subject CA1 area
dc.subject DG area.
dc.title THE PROTECTIVE EFFECTS OF CHRONIC CAFFEINE TREATMENT ON THE COGNITIVE FUNCTION AND SYNAPTIC PLASTICITY IN ACUTE SLEEP DEPRIVATION
dc.date.updated 2012-09-27T15:55:03Z
dc.identifier.slug 10657/ETD-UH-2010-05-31
dc.type.material text *
dc.type.genre thesis *
thesis.degree.name Pharmacology (Ph.D.)
thesis.degree.level Doctoral
thesis.degree.discipline Pharmacology
thesis.degree.grantor University of Houston
thesis.degree.department Pharmacological and Pharmaceutical Sciences
dc.contributor.committeeMember Lau, Vincent
dc.contributor.committeeMember Hussain, Tahir
dc.contributor.committeeMember Leasure, J Leigh
dc.contributor.committeeMember Aleisa, Abdulaziz

Files in this item

This item appears in the following Collection(s)

Show simple item record