The relationship between nitric oxide synthase (NOS) and cyclooxygenase (COX) in the control of cervical ripening and parturition

DSpace/Manakin Repository

The relationship between nitric oxide synthase (NOS) and cyclooxygenase (COX) in the control of cervical ripening and parturition

Show simple item record


dc.contributor.advisor Robert E. Garfield, Ph.D., Supervisor en_US
dc.creator Stephen Gureasko Marx en_US
dc.date.accessioned 2011-12-20T16:04:28Z
dc.date.available 2008-04-03 en_US
dc.date.available 2011-12-20T16:04:28Z
dc.date.created 2005-03-30 en_US
dc.date.issued 2005-03-14 en_US
dc.identifier.other etd-03302005-094718 en_US
dc.identifier.uri http://hdl.handle.net/2152.3/73
dc.description.abstract Stephen G. Marx\r\nThe University of Texas Medical Branch at Galveston, April 2005\r\n\r\nSupervisor: Robert E. Garfield\r\n\r\nThe purpose of these studies is to examine if there is relationship between iNOS and COX-2 in the control of cervical ripening and parturition. Cervices were obtained from estrus and timed pregnant Sprague-Dawley rats (n = 4-10 per group) under normal conditions; or after treating with LPS (100ƒÝg i.p.), Onapristone (3mg/rat), progesterone (2.5 mg, twice daily), L-NAME (50mg/day), or SNP (0.3mg/rat). Collagen changes were measured and visualized with the picrosirius polarization method. Expression of iNOS and COX-2 mRNA was determined using RT-PCR. Immunohistochemistry (IHS) was performed for localization of the iNOS and COX-2 enzymes (significance: P<0.05). Picrosirius polarization showed a decrease in the organization and birefringence of the cervical collagen from the non-pregnant state through pregnancy and is supported by changes in the luminosity (P<0.001). The iNOS and COX-2 enzymes were mainly localized in the cervical muscle with labeling also in the vascular smooth muscle and epithelium. Under normal term pregnant conditions, iNOS mRNA levels decrease as COX-2 mRNA levels increased demonstrating an inverse correlation (Spearman r = -0.497; P = 0.00295). Onapristone stimulated preterm labor and/or birth causing a parallel increase in iNOS and COX-2 mRNA demonstrating a positive correlation (Spearman r = 0.456; P = 0.03). Progesterone prolonged pregnancy stimulating a decrease in the iNOS and COX-2 (P=0.036) mRNA. In comparing term to preterm laboring conditions, there is a significant increase in the iNOS mRNA (P=0.004) but not the COX-2 mRNA. LPS enhanced the iNOS mRNA (P<0.001) but had no effect on the COX-2 mRNA. L-NAME had no effect on the COX-2 or iNOS mRNA. SNP decreased the COX-2 and iNOS with the decrease in the iNOS being significant (P=0.007). In conclusion, under normal term pregnant conditions iNOS and COX-2 play an important role in regulating cervical ripening and parturition but the pathways appear to act independently of one another in regulating iNOS and COX-2 expression at the mRNA level. Under preterm laboring conditions, when NO is up regulated and/or over expressed, there may be an interaction between the NO and PG pathways in the control of cervical ripening and parturition. \r\n\r\n en_US
dc.format.medium electronic en_US
dc.language.iso eng en_US
dc.rights Copyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works. en_US
dc.subject prostagladins en_US
dc.subject prolongation of pregnancy en_US
dc.subject preterm labor en_US
dc.subject nitric oxide en_US
dc.subject iNOS en_US
dc.subject COX2 en_US
dc.subject cervix en_US
dc.title The relationship between nitric oxide synthase (NOS) and cyclooxygenase (COX) in the control of cervical ripening and parturition en_US
dc.type.material text en_US
dc.type.genre dissertation en_US
thesis.degree.name PhD en_US
thesis.degree.level Doctoral en_US
thesis.degree.grantor The University of Texas Medical Branch en_US
thesis.degree.department Cell Biology en_US
dc.contributor.committeeMember Yurij Vedernikov, M.D., Ph.D. en_US
dc.contributor.committeeMember Thomas Collins, Ph.D. en_US
dc.contributor.committeeMember Randall Given, Ph.D. en_US
dc.contributor.committeeMember Gwendoly V. Childs, Ph.D. en_US
dc.contributor.committeeMember George Saade, M.D. en_US

Files in this item

Files Size Format View
Marx.pdf 1.367Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account