Show simple item record

dc.contributor.advisorJohnny Petersonen_US
dc.creatorBryan Thomas Gnadeen_US
dc.date.accessioned2011-12-20T16:04:17Z
dc.date.available2010-09-28en_US
dc.date.available2011-12-20T16:04:17Z
dc.date.created2010-03-11en_US
dc.date.issued2010-01-11en_US
dc.identifier.otheretd-03112010-180727en_US
dc.identifier.urihttp://hdl.handle.net/2152.3/40
dc.description.abstractBacillus anthracis is a gram-positive spore-forming rod capable of causing cutaneous, gastrointestinal and inhalational anthrax. It has a number of virulence factors of which, the two toxins are of great importance. Lethal toxin is a zinc metaloprotease that cleaves the N terminus of the mitogen-activated protein kinase (MAPK) kinase family 1-7, with the exception of MEK5. This kinase family is responsible for activating the mitogen-activated protein kinase (MAPK) cascade. This cascade includes extracellular signal-regulated protein kinases (ERK), c-Jun NH2-terminal kinases (JNK) and p38 kinases. The disruption of these signaling pathways has a number of deleterious downstream effects that vary by cell type. Edema factor is a powerful calmodulin-dependent adenylyl cyclase that forms 3’,5’-adenosine monophosphate (cAMP) from adenosine triphosphate (ATP). This enzymatic reaction causes an increase in intracellular cyclic AMP (cAMP) in host cells. As cAMP is a prominent second messenger in cellular signaling, edema toxin has a wide array of effects on numerous cell types and functions. \r\nTo determine the effects of the anthrax toxins on the adaptive immune response, B lymphocytes were exposed to LeTx or EdTx in vitro. LeTx and EdTx both inhibit B cell activation in different manners. LeTx inhibited B cell proliferation but not migration, while EdTx inhibited B cell migration but not proliferation. LeTx and EdTx altered expression patterns of B cell activation markers. EdTx inhibited MIP-1α and MIP-1β while enhancing IL-6 production. Previously unseen in any cell type EdTx was demonstrated to be cytotoxic to naïve B cells.\r\nThe research presented in this report illustrates the inhibitory effects of LeTx and EdTx on B lymphocytes, providing valuable insight into the immunoevasion tactics of B. anthracis.\r\nen_US
dc.format.mediumelectronicen_US
dc.language.isoengen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectlethal toxinen_US
dc.subjectedema toxinen_US
dc.subjectbacillus anthracisen_US
dc.subjectB cellen_US
dc.subjectanthrax toxinen_US
dc.subjectanthraxen_US
dc.titleAnthrax toxin effects on B lymphocyte functionen_US
dc.type.materialtexten_US
dc.type.genredissertationen_US
thesis.degree.namePhDen_US
thesis.degree.levelDoctoralen_US
thesis.degree.grantorThe University of Texas Medical Branchen_US
thesis.degree.departmentMicrobiology and Immunologyen_US
dc.contributor.committeeMemberRolf Konigen_US
dc.contributor.committeeMemberGustavo Valbuenaen_US
dc.contributor.committeeMemberDavid Craften_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record