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Abstract:
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The DNA-damaging potential of the compound 1,2-diinethylhydrazine (DMH) was investigated using the Bacillus subtilis tester systems, which included the DNA repair assay, DNA-DMH binding studies, and DNA transformation. The compound was shown to specifically inhibit the growth of recombination deficient strains of B^. subtilis more than the wild type strain. For DNA-damaging activity, the pH had to be above 6.5. Below this pH, the compound did not appear to be active. Activation with rat-liver homogenate did not increase the DNA-damaging activity. The absorption spectrum of DNA isolated from the recombination deficient strain, mc-1, was altered when the DNA was challenged 2J1 vivo with DMH but not when challenged iji vitro. Transformation with treated DNA from this strain was reduced compared to DNA isolated from an unchallenged culture. |