Optimization Of Methods For In-vitro Expansion And Cryopreservation Of Mammalian Stem Cells
| Title: | Optimization Of Methods For In-vitro Expansion And Cryopreservation Of Mammalian Stem Cells |
| Author: | Balachandran, Harikrishnan |
| Abstract: | The recent advances in the field of Tissue Engineering have given rise to the need for a tremendous quantity of cells . The search for and use of an appropriate multipotent or pluripotent stem cells in tissue engineering is an emerging concept . Mesenchymal stem cells hold a very great deal of promise because of their capacity to self -renew and to differentiate into various lineages . These cells are very low in number in the body but their in vitro expansion capacity is very unique . Taking into consideration the lack of uniform approach for expansion of these MSCs and storing these cells for future use , in this work focus is laid on identifying the optimal culture and storage conditions for MSCs . The data obtained from this study indicates that the media and the quantity of the serum play a very important role in the expansion . Addition of HB -EGF has been shown to increase the proliferation . The current viability rates , post recovery vary according to the cell type used . This gives rise to the need for analyzing various cells . In this thesis the experiments are based on two cell lines models . The viability rates obtained as the end result are used as a standard for optimizing the process for all mammalian cells . A pilot study involving rat mesenchymal stem cells was also performed to evaluate if the cryopreservation of stem cells is similar to the mammalian cell models . The results show that the viability rate of the cells post cryopreservation is high when there is 5 % of DMSO in the freeze media and when the step -by -step slow cooling process is used . In conclusion , this thesis defines the optimal culture conditions for the expansion of MSCs in high numbers and storage of these cells for subsequent therapeutic approaches . |
| URI: |
http : / /hdl .handle .net /10106 /526
http : / /hdl .handle .net /10106 /526 |
| Date: | 2007-08-23 |
Citation
(May 2006).
Optimization Of Methods For In-vitro Expansion And Cryopreservation Of Mammalian Stem Cells.
University of Texas at Arlington. Available electronically from
http : / /hdl .handle .net /10106 /526
.
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