A Hydroponic Approach to Evaluate Salt Tolerance in Cotton

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Title: A Hydroponic Approach to Evaluate Salt Tolerance in Cotton
Author: Castillo, Natalia
Abstract: Interests in developing salt tolerant cultivars have intensified as the result of an increasing population and environmental constraints that limit crop productivity . New agronomic models have been designed to address the new challenges . Some include the development of a salt tolerant crop by evaluation and selection of tolerant germplasm , hybridization with selected germplasm , and development of inbred lines through pedigree selection or backcross , and testing of selected inbred lines . Many wild accessions in cotton have been found in many areas in the world and made accessible for research . Plant breeders are trying to produce new cotton varieties that can withstand the excessive salt concentrations found in the soil and in the water since salinity has become a limiting factor in crop agriculture . For farmers the abiotic stress due to salinity limits yield by inhibiting germination , flowering , and the development of vegetative branches and fruiting branches . Other concerns of plant breeders are abscission of cotton bolls , a reduction in cellulose deposition , and that both photosynthesis and stoma conductance are affected during the breakdown and conversion into carbohydrates . Preliminary studies conducted in 2006 , 2007 , 2008 , and 2009 show one wild accession , TX 307 to be significantly more tolerant than others when plants were treated with NaCl . Since 2010 , the focus of this research has been to use the hydroponic system as a screening method to evaluate and compare responses among cotton genotypes for salt tolerance characteristics to salinity stress . The wild cotton accession TX 307 was used as a control line to compare to three commercial cultivars ‘FM 989’ (PI603956 , PVP9800259 ) , ‘DP 491’ (PI618609 , PVP 200100159 , US2003 /0229928AI ) , and ‘Coker 312’ (PI529278 , PVP7200100 ) ; and two transgenic lines Avp68 and Avp86 . Coker 312 was the cultivar used for the transformation and regeneration of Avp68 and Avp86 transgenic lines . Responses were evaluated at different salt concentrations (dose response ) and at the same concentrations over time . The study was conducted under greenhouse conditions at the AgriLife Research and Extension Center in Lubbock , Texas . Experimental design was a randomized split block with four replications , four treatments , and ten plants /treatment sampling error . Significant differences (p < 0 .05 ) were found during the dose response screening study , however , no significant response genotype interactions over time was observed . The hydroponic system was perhaps not the best method for evaluating response differences at later plant development stages because of possible premature plant death , but it was an effective screening method to isolate individual plants with respect to morphological and physiological responses to salinity stress . The system could also be used as an assessment tool to identify contrasting phenotypes at high salt concentrations early in plant development .
URI: http : / /hdl .handle .net /2346 /ETD -TTU -2011 -08 -1878
Date: 2011-08

Citation

A Hydroponic Approach to Evaluate Salt Tolerance in Cotton. Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /ETD -TTU -2011 -08 -1878 .

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