Identification and characterization of digalacturonic acid uptake mutants in Erwinia chrysanthemi

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Title: Identification and characterization of digalacturonic acid uptake mutants in Erwinia chrysanthemi
Author: Xiang, Zhixin
Abstract: Erwinia chrysanthemi produces several plant cell wall degrading enzymes to cause soft -rot disease in plants . Digalacturonic acid (dGA ) is one of the major products released due to the action of pectolytic enzymes on polygalacturonic acid . Using chemical and transposon mutagenesis , clones defective in digalacturonic acid utilization were generated . Two mutants were identified by having reduced growth on dGA but normal growth on glycerol and GA . These mutants had normal levels of pectin degrading enzymes as well as normal levels of the intracellular enzymeoligogalacturonate lyase . Further analysis of these mutants revealed that they were partially defective in transport of the molecule . These dGA -uptake mutants were unable to cause maceration of potato tuber tissue . These mutants will be useful in cloning the gene (s ) encoding the dGA transport protein (s ) .
URI: http : / /hdl .handle .net /2346 /8641
Date: 1996-05

Citation

Xiang, Zhixin Identification and characterization of digalacturonic acid uptake mutants in Erwinia chrysanthemi. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /8641 .

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