Nano-mechanical studies of prostate cancer cells using atomic force microscopy

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Title: Nano-mechanical studies of prostate cancer cells using atomic force microscopy
Author: Bastatas, Lyndon D
Abstract: Nano -mechanical properties of prostate cancer cells with different metastatic potentials were investigated , in vitro , to shed light on the issue whether those properties could be utilized as indicators of their aggressiveness . Experimentations involved atomic force microscopy -based indenting and force mapping experiments acquired at LNCaP (lowly metastatic ) and CL -1 (highly metastatic ) prostate cancer cells that were cultured on plain glass and nano -scaffolds . Various models were applied to extract the mechanical information pertaining to the elasticity , adhesion and topography of the cells . Deviating from the general perspective for metastasis , the elasticity measurements reveal that the elastic modulus of highly metastatic cancer cells , CL -1 , is about twice as high (i .e . , stiffer ) as the elastic modulus of lowly metastatic cancer cells , LNCaP , both at the center of cells’ body and lamellipodia . The two -dimensional maps of adhesion property , in conjunction with the results from the experiments conducted on the nano -scaffolds , reveal that the stiffer cells , CL -1 , are more adherent than softer cells , LNCaP . These findings are consistent to the reports observed at in vitro nano -mechanical studies that cultured adherent cells are less deformable than the non -adherent ones . We postulate that the enhanced adhesion generates larger cortical tension that leads to higher elastic modulus observed in CL -1 .
URI: http : / /hdl .handle .net /2346 /45368
Date: 2012-05

Citation

Nano-mechanical studies of prostate cancer cells using atomic force microscopy. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /45368 .

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