Utilizing functionalized nano-patterned surfaces as a clue to cell metastasis in prostate and breast cancers

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Title: Utilizing functionalized nano-patterned surfaces as a clue to cell metastasis in prostate and breast cancers
Author: Matthews, James
Abstract: There is a direct relation between the survival of a patient diagnosed with prostate or breast cancer and the metastatic potential of the patient’s cancer . For low metastatic potentials , the survival rates were near 100 % , but for higher metastatic potentials , the survival rates were drastically lower . It is therefore extremely important to prognose metastatic potentials . In this study we investigated whether the behaviors of cancer cells responding to our state of the art nano -patterns differ by the metastatic potential of the cancer cells . We have used lowly (LNCaP ) and highly (CL -1 ) metastatic human prostate can - cer cells[1] and lowly (MCF -7 ) and highly (MB231 ) metastatic breast cancer cells[2] . A surface functionalization study was then performed first on uniform gold and glass surfaces , then on gold nano -patterned surfaces made by nano -sphere lithography . The nano -spheres in diameter of 200nm to 800nm were used and the gold surfaces were functionalized with fibronectin (FN ) . The surface functionalization is confirmed through XPS analysis . The CL -1 , MCF -7 , and MB231 cells show similar prolifera - tion on all surfaces regardless of the presence of FN , whereas LNCaP show a clear preference for FN coated surfaces . The proliferation of the LNCaP was reduced when grown on finer nano -scaffolds , but the more aggressive CL -1 , MB231 , and MCF -7 cells show an abnormal proliferation regardless of pattern size . The difference in adhesion is intrinsic and was verified through dual fluorescent imaging . Clear co -localization of actin -vinculin were found on CL -1 , MCF -7 , and MB231 . However LNCaP cells showed the co -localization of actin and vinculin only on the tips of the cells . These results provide vital clues to the bio -mechanical differences between the cancer cells with different metastatic potential .
URI: http : / /hdl .handle .net /2346 /45148
Date: 2012-05

Citation

Utilizing functionalized nano-patterned surfaces as a clue to cell metastasis in prostate and breast cancers. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /45148 .

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