Callus induction, maintenance and regeneration of interspecific allium hybrids

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Title: Callus induction, maintenance and regeneration of interspecific allium hybrids
Author: Lu, Chung-cheng
Abstract: A culture system for plant regeneration via somatic embryogenesis of interspecific F1 hybrids was developed . Inflorescence and basal plate of 25 genotypes of interspecific Allium fistulosum x ^ . cepa F . hybrids were cultured . Basal BDS medium containing 0 .75 mg /1 picloram and 2 .0 mg /a BA was ideal to induce and maintain callus culture . Callus regeneration was enhanced by proline and vitamins . Embryogenesis was regenerated on 2 media from 6 out of 25 genotypes of basal plate explant . Inflorescence explant contained higher regeneration potential and regenerated from 15 out of 16 treatments . Somaclonal variants were obtained . No karyotypic analyses were conducted so no putative chromosomal abnormalities occurring during culture were observed . Furthermore , variable isozyme banding patterns were found in Adh and Pgi .
URI: http : / /hdl .handle .net /2346 /19299
Date: 1987-08

Citation

Callus induction, maintenance and regeneration of interspecific allium hybrids. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /19299 .

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