Bacterial degradation of diesel fuel using pure and mixed cultures

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dc.creator Tasker , Rushikesh
dc.date 2011 -02 -18T22 :54 :06Z
dc.date 2012 -06 -01T14 :30 :04Z
dc.date 2011 -02 -18T22 :54 :06Z
dc.date 2012 -06 -01T14 :30 :04Z
dc.date 2011 -02 -18T22 :54 :06Z
dc.date 1988 -01 -01
dc.date.accessioned 2012 -11 -29T19 :47 :13Z
dc.date.available 2012 -11 -29T19 :47 :13Z
dc.date.issued 2012 -11 -29
dc.identifier http : / /hdl .handle .net /2346 /19058
dc.identifier.uri http : / /hdl .handle .net /2346 /19058
dc.description Eight bacteria capable of using diesel fuel as the sole carbon source were isolated from both a bunker oil -saturated soil sample and from a gasoline -contaminated water sample . The three isolates from the soil were identified as Pseudomonas vesicularis . And two strains of Pseudomonas aeruginosa . The five water isolates were identified as Achromobacter xvloxidans . Achromobacter sp . , Aeromonas sp . , Pseudomonas sp . , and Pseudomonas vesicularis . The components of diesel fuel degraded by the isolates over a three week period were determined by capillary gas chromatography . The growth curves of the isolates in 1 .5 % yeast extract and in basal salts medium with diesel as carbon source were obtained . The generation times of each isolate in 1 .5 % yeast extract and in basal salts medium with diesel as carbon source were calculated and the significant differences in generations times were determined . Two sets of mixed cultures were used , one consisting of the three soil isolates and the other of the five water isolates , to determine the effect of mixed cultures on diesel over a three week period . The mixed cultures were found to be more efficient than the pure cultures at reducing the total number of fractions of diesel .
dc.language en _US
dc.publisher Texas Tech University
dc.rights unrestricted
dc.subject Petroleum - - Biodegradation
dc.subject Petroleum - - Microbiology
dc.subject Oil pollution of soils
dc.title Bacterial degradation of diesel fuel using pure and mixed cultures
dc.type Electronic Thesis

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Bacterial degradation of diesel fuel using pure and mixed cultures. Available electronically from http : / /hdl .handle .net /2346 /19058 .

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