Bacterial degradation of diesel fuel using pure and mixed cultures

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Title: Bacterial degradation of diesel fuel using pure and mixed cultures
Author: Tasker, Rushikesh
Abstract: Eight bacteria capable of using diesel fuel as the sole carbon source were isolated from both a bunker oil -saturated soil sample and from a gasoline -contaminated water sample . The three isolates from the soil were identified as Pseudomonas vesicularis . And two strains of Pseudomonas aeruginosa . The five water isolates were identified as Achromobacter xvloxidans . Achromobacter sp . , Aeromonas sp . , Pseudomonas sp . , and Pseudomonas vesicularis . The components of diesel fuel degraded by the isolates over a three week period were determined by capillary gas chromatography . The growth curves of the isolates in 1 .5 % yeast extract and in basal salts medium with diesel as carbon source were obtained . The generation times of each isolate in 1 .5 % yeast extract and in basal salts medium with diesel as carbon source were calculated and the significant differences in generations times were determined . Two sets of mixed cultures were used , one consisting of the three soil isolates and the other of the five water isolates , to determine the effect of mixed cultures on diesel over a three week period . The mixed cultures were found to be more efficient than the pure cultures at reducing the total number of fractions of diesel .
URI: http : / /hdl .handle .net /2346 /19058
Date: 1988-08

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Bacterial degradation of diesel fuel using pure and mixed cultures. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /19058 .

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