Early indications of breed differences for cryopreservation of embryos in cattle

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Title: Early indications of breed differences for cryopreservation of embryos in cattle
Author: Weathers, Julie
Abstract: Cryopreservation is considered state of the art procedure to store embryos in most industry settings . However , some species (i .e . humans ) do not survive the freezing /thawing process well ; additionally , some breeds within successful species do not survive this process well either (i .e . Jersey cattle ) ; therefore , more research is needed to perfect the process . While the cryopreservation process assumes that water is conserved across species , there is no proof this assumption is correct . Thus , any significant change in the chemical constituents of embryos could identify the problems with that species’ or breed’s freezing and thawing success rates . Therefore , the objective of this study was to determine if embryos from different breeds within the same species demonstrate any significantly different in chemical compositions . In these experiments , a unique modified specific gravity chamber was created in order to estimate the weight of preimplantation embryos from varying cattle breeds . Once the system had been standardized with beads of know size and weight , day six embryos from Jersey and beef cattle were processed through the system and the rate of descent plotted against the standard curve to estimate embryo weight . Additionally , embryos from both groups were fixed and sectioned for electron microscopy , and random micrographs used to quantify lipid content . Data analysis was performed on SPSS version 12 with an intra -assay CV being run between standard curves and a Student’s T -Test for lipid quantification . Embryos of each breed were amazingly consistent in time of descent . However , the estimated weight of Jersey embryos appeared to be significantly lighter weight than beef cattle embryos (P < .001 ) . Additionally , electron microscopy suggests the Jersey embryos contain greater amounts of lipid droplets at the same stage of development (P < .001 ) . These data represent the first true estimation of embryo weight at this stage of development . Moreover , the data suggest these differences may be due solely to the number lipid droplets found within the cells . Further study will be needed to determine if knowing lipid content of embryos of individual breeds or species will allow improvement in cryopreservation success for that group .
URI: http : / /hdl .handle .net /2346 /18883
Date: 2008-08

Citation

Early indications of breed differences for cryopreservation of embryos in cattle. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /18883 .

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