A method for identifying proteins involved in heat shock protein secretion in Saccharomyces cerevisiae

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Title: A method for identifying proteins involved in heat shock protein secretion in Saccharomyces cerevisiae
Author: Crum, Charles Douglas
Abstract: Yeasts have made a massive contribution to our lives in a variety of ways . They have been used for brewing , baking , pharmaceuticals and other industrial processes for a number of reasons . They possess relatively simple growth requirements and can be cultured easiy . The conservation of most biochemical activities throughout a wide range of organisms has allowed for the use of S . cerevisiae as a model eukaryote which has contributed immensely to our understanding of cell biology . S . cerevisiae exists in a haploid or diploid state and can reproduce both asexually and sexually , having both the a and a mating types . It has a genome that has sixteen linear chromosomes and has been classified in the ascomycetes group of fungi . It is generally considered nonpathogenic except in a very small number of susceptible individuals . The entire genome of S . cerevisiae has been sequenced and is available on various public databases . The Saccharomyces proteome is currently being mapped , which covers the entire range of proteins and thier function . All of these factors allow us to use S . cerevisiae as a model for studying many processes of cellular function including secretion through temperature sensitive mutants , cellular and nuclear organization as well as protein function , for example , one model has utilized the Saccharomyces invertase protein as a marker for the external localization of a gene fused to a hybrid transcript (51 ) . Being a nonpathogenic model organism allows us to utilize the qualities of S . cerevisiae to learn about other pathogenic organism including Candida albicans , C . dublienensis . Cryptococcoccus neoformans and other eukaryotic pathogenic fungi .
URI: http : / /hdl .handle .net /2346 /18551
Date: 2001-12

Citation

A method for identifying proteins involved in heat shock protein secretion in Saccharomyces cerevisiae. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /18551 .

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