Effects of oxyntomodulin on intestinal adaptation in the rat

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Title: Effects of oxyntomodulin on intestinal adaptation in the rat
Author: Zhu, Zhuochen
Abstract: Enteroglucagon (EG ) has long been suggested to be an enterotrophic (mucosal growth ) factor based on circumstantial evidence . This study evaluated the effects of - vivo infusions of synthetic rat oxyntomodulin (OXN , a bioactive form of EG ) and glucagon on intestinal adaptation in rats . Osmotic minipumps which deliver peptides or saline intravenously for up to two weeks were implanted into rats fed with either lab chow or a carbohydrate -free diet . At sacrifice , the entire small intestine was removed , and glucose and proline uptake across the brush border was measured using the everted sleeve method . Gut morphology was also measured to assess effects on growth . Surprisingly , OXN infusion had no effect on intestinal growth , despite the putative correlation between EG and enterocyte proliferation . In contrast , infusion of OXN increased active glucose uptake by 13 -48 % in proximal gut , and by nearly 300 % in the ileum . However , OXN showed no effect on proline uptake . On the other hand , glucagon infusion increased active glucose uptake by 100 -150 % in the proximal and middle part of the intestine , but only showed modest effect on the distal gut . Similarly , glucagon infusion had no effects on proline uptake or intestinal growth . These results were affected by diet since rats fed with lab chow and infused with lower dose of OXN showed no effects . This study demonstrates for the first time that OXN or glucagon increases intestinal nutrient absorption by specifically stimulating glucose uptake . These results do not support the long -standing hypothesis of OXN as a gut growth factor .
URI: http : / /hdl .handle .net /2346 /11859
Date: 1995-05

Citation

Zhu, Zhuochen Effects of oxyntomodulin on intestinal adaptation in the rat. Master's thesis, Texas Tech University. Available electronically from http : / /hdl .handle .net /2346 /11859 .

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