Comparative venomic studies of the western cottonmouth (Agkistrodon Piscivorus Leucostoma) and the western diamondback rattlesnake (Crotalus Atrox)

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dc.contributor.advisor Osterhout , John J . Pleen , Joseph
dc.contributor.other Angelo State University . Department of Biology .
dc.contributor.committeeMember Jones , Crosby W .
dc.contributor.committeeMember Dixon , Michael T .
dc.contributor.committeeMember May , Brian J .
dc.creator Pleen , Joseph J 2012 -10 -05T17 :30 :06Z 2014 -02 -19T21 :57 :05Z 2012 -10 -05T17 :30 :06Z 2014 -02 -19T21 :57 :05Z 2012 -07 -18 2012 -08 -10 2012 -10 -04
dc.identifier.uri http : / /hdl .handle .net /2346 .1 /30024
dc.description.abstract The ability to identify and classify individual venomic variation is essential to understanding intraspecific and intergenus variation of snake venom proteomes (venomes ) and useful for developing more toxin specific antivenom . The study of individual snake venomes using new methodologies is needed to improve our understanding of the ontogenic , ecological , and evolutionary forces that shape venom as well as provide a potential source for new therapeutic agents . The methodological groundwork for the intergenus and intraspecific comparison of the venom proteomes of the western cottonmouth (Agkistrodon piscivorus leucostoma ) and the western diamondback rattlesnake (Crotalus atrox ) is presented in this study . The use of multiple orthogonal techniques including nano -flow ultraperformance liquid chromatography elevated energy level mass spectroscopy (nanoUPLC -MSE ) enabled the identification of 74 unique proteins with an average protein sequence coverage of 39 .3 % . Size exclusion chromatography , reverse phase high performance liquid chromatography (RPHPLC ) , matrix -assisted laser desorption /ionization tandem time of flight mass spectrometry (MALDI -TOF /TOF -MS ) and de novo sequencing revealed 7 novel peptides . Quantitation of mass spectra data also showed that relative protein abundance differed considerably between A . p . leucostoma and C . atrox . In summary , the proteomics data generated have served to expand the current understanding of both the A . p . leucostoma venome and C . atrox venome and helped to build a framework for future venomic study . en_US
dc.format.mimetype application /pdf
dc.language.iso en _US en_US
dc.subject western cotttonmouth en_US
dc.subject western diamondback en_US
dc.subject venom en_US
dc.subject venomic variation en_US
dc.title Comparative venomic studies of the western cottonmouth (Agkistrodon Piscivorus Leucostoma ) and the western diamondback rattlesnake (Crotalus Atrox ) en_US
dc.type Thesis en_US
dc.type.genre Thesis
dc.type.material text M .S . Masters Biology Department of Biology Angelo State University


Pleen, Joseph Comparative venomic studies of the western cottonmouth (Agkistrodon Piscivorus Leucostoma) and the western diamondback rattlesnake (Crotalus Atrox). Master's thesis, Angelo State University. Available electronically from http : / /hdl .handle .net /2346 .1 /30024 .

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