Comparative venomic studies of the western cottonmouth (Agkistrodon Piscivorus Leucostoma) and the western diamondback rattlesnake (Crotalus Atrox)

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Title: Comparative venomic studies of the western cottonmouth (Agkistrodon Piscivorus Leucostoma) and the western diamondback rattlesnake (Crotalus Atrox)
Author: Pleen, Joseph
Abstract: The ability to identify and classify individual venomic variation is essential to understanding intraspecific and intergenus variation of snake venom proteomes (venomes ) and useful for developing more toxin specific antivenom . The study of individual snake venomes using new methodologies is needed to improve our understanding of the ontogenic , ecological , and evolutionary forces that shape venom as well as provide a potential source for new therapeutic agents . The methodological groundwork for the intergenus and intraspecific comparison of the venom proteomes of the western cottonmouth (Agkistrodon piscivorus leucostoma ) and the western diamondback rattlesnake (Crotalus atrox ) is presented in this study . The use of multiple orthogonal techniques including nano -flow ultraperformance liquid chromatography elevated energy level mass spectroscopy (nanoUPLC -MSE ) enabled the identification of 74 unique proteins with an average protein sequence coverage of 39 .3 % . Size exclusion chromatography , reverse phase high performance liquid chromatography (RPHPLC ) , matrix -assisted laser desorption /ionization tandem time of flight mass spectrometry (MALDI -TOF /TOF -MS ) and de novo sequencing revealed 7 novel peptides . Quantitation of mass spectra data also showed that relative protein abundance differed considerably between A . p . leucostoma and C . atrox . In summary , the proteomics data generated have served to expand the current understanding of both the A . p . leucostoma venome and C . atrox venome and helped to build a framework for future venomic study .
URI: http : / /hdl .handle .net /2346 .1 /30024
Date: 2012-08-10

Citation

Pleen, Joseph Comparative venomic studies of the western cottonmouth (Agkistrodon Piscivorus Leucostoma) and the western diamondback rattlesnake (Crotalus Atrox). Master's thesis, Angelo State University. Available electronically from http : / /hdl .handle .net /2346 .1 /30024 .

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