Phylogeography of the cottonmouth, Agkistrodon piscivorus, using AFLP and venom protein profiles.

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Title: Phylogeography of the cottonmouth, Agkistrodon piscivorus, using AFLP and venom protein profiles.
Author: Strickland, Jason L.
Abstract: The objective of this study was to examine population structure in cottonmouths (Agkistrodon piscivorus ) using Amplified Fragment Length Polymorphism (AFLP ) and compare genetic and venom protein profiles in Texas . AFLP profiles using 622 fragments were generated for 105 individuals to understand the level of variation within Agkistrodon . In Texas , there was a significant lack of gene flow detected and support for the isolation of Concho Valley individuals . Cottonmouths showed the greatest genetic variation when compared to other Agkistrodon species but there was not complete support for two species of cottonmouths as currently proposed . RP -HPLC was used to examine venom protein profiles in 86 Texas cottonmouths . Relative peak heights were analyzed using PCA and the MANOVA demonstrated separation of populations based on profiles (p <0 .001 ) . Genetic and venom variation did not follow the same pattern indicating that there may be other selection pressures acting on the venom proteins .
URI: http : / /hdl .handle .net /2346 .1 /17
Date: 2011-05-25

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Strickland, Jason L. Phylogeography of the cottonmouth, Agkistrodon piscivorus, using AFLP and venom protein profiles.. Master's thesis, Angelo State University. Available electronically from http : / /hdl .handle .net /2346 .1 /17 .

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