| dc.contributor.advisor |
Mehdy , Mona Cynthia , 1955 - |
|
| dc.contributor.committeeMember |
Sathasivan , Kanagasabapathi |
|
| dc.creator |
Byrwa , Brian Christopher |
|
| dc.date.accessioned |
2012 -02 -27T21 :17 :53Z |
|
| dc.date.available |
2012 -02 -27T21 :17 :53Z |
|
| dc.date.created |
2011 -08 |
|
| dc.date.issued |
2012 -02 -27 |
|
| dc.date.submitted |
August 2011 |
|
| dc.identifier.uri |
http : / /hdl .handle .net /2152 /ETD -UT -2011 -08 -4367 |
|
| dc.description.abstract |
Considering the nature of increasing global temperatures associated with elevated atmospheric carbon dioxide levels as a result of increased demand for energy , it is notable to consider viable options to reduce the strain that these increased carbon dioxide emissions are having on the overall impact of the global climate . Phaeodactylum tricornutum , a marine phytoplankton may be utilized to this end . Its unique ability to increase lipid production under environmental stress conditions , in particular those lipids that can easily be converted into biodiesel , make it an ideal candidate for this use . Here , we examine the effects of trans trans 2 ,4 decadienal (or DD for short ) , an aldehyde that is known to induce cell death in the diatom at high concentrations , as they relate to changes in the lipid biosynthesis pathway . 100 ml Axenic cultures of the diatom P . tricornutum were grown to exponential stage , harvested and treated with decadienal at a concentration of 5[mu]g /ml to determine effects on lipid production after 24 hours . Qualitative analysis undertaken using Nile red staining of treated and untreated cells indicated increased fluorescence of treated cells compared to unstained water controls , however this increase may not be attributable to increased lipid production due to the fact that cells were unfixed and must be verified through other means . Initial attempts to verify this finding through thin layer chromatography and qPCR were inconclusive . |
|
| dc.format.mimetype |
application /pdf |
|
| dc.language.iso |
eng |
|
| dc.subject |
Phaeodactylum tricornutum |
|
| dc.subject |
Trans trans 2 ,4 decadienal |
|
| dc.title |
Lipid analysis of Phaeodactylum tricornutum in response to trans trans 2 ,4 decadienal stress |
|
| dc.description.department |
Science and Mathematics Education |
|
| dc.type.genre |
thesis |
* |
| dc.type.material |
text |
* |
| thesis.degree.name |
Master of Arts |
|
| thesis.degree.level |
Masters |
|
| thesis.degree.discipline |
Science Education |
|
| thesis.degree.grantor |
University of Texas at Austin |
|
| thesis.degree.department |
Science and Mathematics Education |
|
| dc.date.updated |
2012 -02 -27T21 :18 :00Z |
|
| dc.identifier.slug |
2152 /ETD -UT -2011 -08 -4367 |
|