Developing a diagnostic tool for acyl carrier proteins through trypsinolysis, reverse-phase chromatography and native chemical ligation

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Title: Developing a diagnostic tool for acyl carrier proteins through trypsinolysis, reverse-phase chromatography and native chemical ligation
Author: Reyes, Graciela, 1957-
Abstract: Polyketide biosynthesis is a field that has had tremendous advances in the past 50 years . The understanding of the mechanisms is updated as investigations delve into domain interactions of these microbial natural products . Although numerous polyketides are known , similarities in the sequence of product generation can be used as templates for further exploration of enzymatic activity . The focus of studies recently has been towards developing protocols to manipulate the natural products resulting in medicinally important manufactured products . This investigation examined the mechanism of the acyl carrier protein (ACP ) module involved in biosynthesis .
URI: http : / /hdl .handle .net /2152 /ETD -UT -2010 -08 -1725
Date: 2011-01-06

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Developing a diagnostic tool for acyl carrier proteins through trypsinolysis, reverse-phase chromatography and native chemical ligation. Master's thesis, University of Texas at Austin. Available electronically from http : / /hdl .handle .net /2152 /ETD -UT -2010 -08 -1725 .

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