| dc.contributor.advisor |
Roy , Krishnendu |
en_US |
| dc.contributor.advisor |
Shi , Li , Ph . D . |
en_US |
| dc.identifier.oclc |
243480370 |
en_US |
| dc.creator |
Glangchai , Luz Cristal Sanchez , 1977 - |
en_US |
| dc.date.accessioned |
2008 -08 -29T00 :17 :00Z |
|
| dc.date.available |
2008 -08 -29T00 :17 :00Z |
|
| dc.date.created |
2008 |
en_US |
| dc.date.issued |
2008 -08 -29T00 :17 :00Z |
|
| dc.identifier.uri |
http : / /hdl .handle .net /2152 /3888 |
|
| dc.description.abstract |
Our ability to precisely manipulate size , shape , and composition of nanoscale carriers is essential for controlling their in -vivo transport , biodistribution , and drug release mechanism . Shape -specific , "smart" nanoparticles that deliver drugs or imaging agents to target tissues primarily in response to disease -specific or physiological signals could significantly improve therapeutic care of complex diseases . Current methods in nanoparticle synthesis do not allow such simultaneous control over particle size , shape , and environmentally -triggered drug release , especially at the sub -100 nm range . In this dissertation , we discuss the development of high -throughput nanofabrication techniques using synthetic and biological macromers (peptides ) to produce highly monodisperse nanoparticles , as well as enzymatically -triggered nanoparticles , of precise sizes and shapes . We evaluated thermal nanoimprint lithography (ThNIL ) and step and flash imprint lithography (SFIL ) as two possible fabrication techniques . We successfully employed ThNIL and SFIL for fabricating nanoparticles and have extensively characterized the SFIL fabrication process , as well as the properties of the imprinted biopolymers . Particles as small as 50 nm were fabricated on silicon wafers and harvested directly into aqueous buffer using a biocompatible , one -step release technique . These methods provide a novel way to fabricate biocompatible nanoparticles with precise size and geometry . Furthermore , we developed an enzyme -degradable material system and demonstrated successful encapsulation and enzyme -triggered release of antibodies and nucleic acids from these imprinted nanoparticles ; thus providing a potential means for disease -controlled delivery of biomolecules . Finally , we evaluated the bioactivity of the encapsulated therapeutics in -vitro . The development of the SFIL method for fabrication of biocompatible nanocarriers has great potential in the drug delivery field for its ability to create monodisperse particles of pre -designed geometry and size , and to incorporate stimulus -responsive release mechanisms . This research provides the potential to broaden the study of how particle size and shape affect the biodistribution of drugs within the body . |
en_US |
| dc.format.medium |
electronic |
en_US |
| dc.language.iso |
eng |
en_US |
| dc.rights |
Copyright © is held by the author . Presentation of this material on
the Libraries' web site by University Libraries , The University of Texas at Austin was made
possible under a limited license grant from the author who has retained all copyrights in
the works . |
en_US |
| dc.subject.lcsh |
Nanotechnology |
en_US |
| dc.subject.lcsh |
Nanoparticles |
en_US |
| dc.subject.lcsh |
Microlithography |
en_US |
| dc.subject.lcsh |
Drug delivery systems |
en_US |
| dc.subject.lcsh |
Peptides |
en_US |
| dc.title |
Nanoimprint lithography based fabrication of size and shape -specific , enzymatically -triggered nanoparticles for drug delivery applications |
en_US |
| dc.description.department |
Biomedical Engineering |
en_US |
| dc.identifier.recnum |
b70661030 |
en_US |
| dc.type.genre |
Thesis |
en_US |
| dc.type.material |
text |
en_US |
| thesis.degree.name |
Doctor of Philosophy |
en_US |
| thesis.degree.level |
Doctoral |
en_US |
| thesis.degree.discipline |
Biomedical Engineering |
en_US |
| thesis.degree.grantor |
The University of Texas at Austin |
en_US |
| thesis.degree.department |
Biomedical Engineering |
en_US |