Plasmon resonance coupling as a tool for detecting epidermal growth factor receptor expression in cancer

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dc.contributor.advisor Sokolov , Konstantin en_US
dc.contributor.advisor Richards -Kortum , Rebecca , 1964 - en_US
dc.identifier.oclc 182561422 en_US
dc.creator Aaron , Jesse Scott , 1979 - en_US
dc.date.accessioned 2008 -08 -28T23 :49 :18Z
dc.date.accessioned 2014 -02 -19T22 :33 :57Z
dc.date.available 2008 -08 -28T23 :49 :18Z
dc.date.available 2014 -02 -19T22 :33 :57Z
dc.date.created 2007 en_US
dc.date.issued 2008 -08 -28T23 :49 :18Z
dc.identifier.uri http : / /hdl .handle .net /2152 /3462
dc.description.abstract Optical molecular imaging has burgeoned into a major field within biomedicine , and technologies that incorporate surface plasmon resonance effects have become a major focus within this field . Plasmon resonance has been defined as the collective oscillation of the conduction band electrons in certain metals (such as gold ) in response to an electric field , such as an impinging wave of light . We show that elastic light scattering due to the plasmon resonance of nanometer -sized gold particles makes them powerful tools for optical imaging of epidermal growth factor receptor (EGFR ) expression - - a major biomarker for carcinogenesis . Optical technologies in general are poised as cheap , flexible ways to aid in diagnosis and treatment of disease . In addition to supplying a bright , stable optical scattering signal and a convenient conjugation platform for targeting molecules , these materials display a unique behavior termed "plasmon coupling" . This term refers to the dramatic optical property changes brought about by the presence of other nearby nanoparticles . These changes include a dramatic red -shifting in their peak plasmon resonance wavelength , as well as a non -linear , per -particle increase in the overall scattered power . We show that such conditions exist in cells and are primarily due to intricate protein trafficking mechanisms as part of the EGFR life -cycle . The observed variations in plasmon coupling can give clues as to the nanoscale organization of these important proteins . In addition , the resulting optical property changes result in a large , molecular -specific contrast enhancement due to the shifting of the resonance closer to the near infrared region , where biological tissues tend to be most transparent . Despite this enhancement , however , many tissues contain large endogenous signals , as well as barriers to delivery of both light and the nanoparticles . As such , we also show an example of a multifaceted approach for further increasing the apparent molecular -specific optical signals in imaging of EGFR expression by using an oscillating magnetic field . This serves to encode the signal from magnetically susceptible plasmonic nanoparticles , making their extraction from the background possible . Overall , the studies presented in this dissertation should serve to stimulate further investigations into a wide variety of technologies , techniques , and applications . en_US
dc.format.medium electronic en_US
dc.language.iso eng en_US
dc.rights Copyright © is held by the author . Presentation of this material on the Libraries' web site by University Libraries , The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works . en_US
dc.subject.lcsh Surface plasmon resonance en_US
dc.subject.lcsh Cancer - -Diagnosis en_US
dc.subject.lcsh Epidermal growth factor en_US
dc.title Plasmon resonance coupling as a tool for detecting epidermal growth factor receptor expression in cancer en_US
dc.description.department Biomedical Engineering en_US
dc.identifier.recnum b69353013 en_US
dc.type.genre Thesis en_US
dc.type.material text en_US
thesis.degree.name Doctor of Philosophy en_US
thesis.degree.level Doctoral en_US
thesis.degree.discipline Biomedical Engineering en_US
thesis.degree.grantor The University of Texas at Austin en_US
thesis.degree.department Biomedical Engineering en_US

Citation

Plasmon resonance coupling as a tool for detecting epidermal growth factor receptor expression in cancer. Doctoral dissertation, The University of Texas at Austin. Available electronically from http : / /hdl .handle .net /2152 /3462 .

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