Characterization of VIBRIO Parahaemolyticus-induced Intestinal inflammation in the mouse

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Title: Characterization of VIBRIO Parahaemolyticus-induced Intestinal inflammation in the mouse
Author: Lee, Olivia
Abstract: The Gram -negative marine bacterium Vibrio parahaemolyticus is a leading cause of gastroenteritis from the consumption of contaminated seafood . Some recent outbreaks are attributed to a rise in ocean temperatures , a trend that is likely to continue as a result of global warming and emphasizes the need to study the virulence mechanisms of the pathogen . V . parahaemolyticus utilizes a type III secretion system to inject effectors that disrupt signal transduction in eukaryotic hosts during infection . Herein , we describe an in vivo model of infection by V . parahaemolyticus using germfree mice . V . parahaemolyticus is recovered from the cecum and colon of mice infected by oral gavage . Mice infected with a pathogenic , wild type strain of V . parahaemolyticus exhibit intestinal inflammation characterized by epithelial damage , submucosal edema , crypt abscess and hyperplasia , and infiltration of the lamina propria by neutrophils . Using mutant strains of V . parahaemolyticus , we have determined that thermostable direct hemolysin , the most well characterized virulence factor produced by V . parahaemolyticus , and T3SS2 , one of the two type III secretion systems present in this bacterium , both contribute to enteropathogenesis . Analysis of the expression of inflammatory cytokines IL -1 ? and IFN -gamma and chemokines KC , MIP -2 alpha , and CXCL -9 reveals that the duration of the inflammatory response is extended in the presence of TDH . In addition to delineating the roles of the virulence factors TDH , type III secretion system 1 , and type III secretion system 2 , in the infection process , we have developed an experimental system that will enable further characterization of type III effectors of V . parahaemolyticus .
URI: http : / /hdl .handle .net /2152 .5 /681
Date: 2009-09-04


Characterization of VIBRIO Parahaemolyticus-induced Intestinal inflammation in the mouse. Graduate School of Biomedical Sciences. Available electronically from http : / /hdl .handle .net /2152 .5 /681 .

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