Adipose-Derived Stromal Cells Contribute To Spinal Cord Repair But Are Not Neural-Crest Derived Stem Cells

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Title: Adipose-Derived Stromal Cells Contribute To Spinal Cord Repair But Are Not Neural-Crest Derived Stem Cells
Author: Wrage, Philip Charles
Abstract: Neurodegeneration and injury to the nervous system are characterized by a loss of neurons - and often supporting glia - at the afflicted site . Neurons of the adult CNS are terminally differentiated , non -mitotic cells that are connected within specific circuits . These characteristics present a challenge to the development of treatments for degeneration or injury of the nervous system . The limited spatial distribution , as well as limited migration and differentiation potentials of adult NSCs , severely restrict the ability of adult NSCs to contribute to repair or regeneration in the wake of injury or degenerative disease progression . Adipose -derived adult stromal (ADAS ) cells have been reported to give rise to cells of both mesodermal and ectodermal origin (e .g . osteocytes , chondrocytes , cardiac myocytes , neurons , and glia ) and are easily harvested and cultured in vitro . Neural crest derived tissues have the extraordinary capacity to give rise to a wide range of tissue types : neurons and glia of the peripheral nervous system , adrenal glands , chondrocytes and osteocytes of the head and neck , smooth muscle cells of the cardiac outflow tract , and melanocytes among others . Given the reported ability of neural crest -derived cells and ADAS cells to give rise to bone , cartilage , muscle , and nerve tissues , I hypothesized that ADAS cells might be neural crest -derived cells that had migrated to the periphery , had remained resident within the adipose tissue of adult mammals , and had maintained early developmental plasticity . This hypothesis was not supported by lineage tracing experiments . Additionally , I found that ADAS cells were not capable of differentiating into functional neurons in vitro or in an in vivo model of spinal cord injury . However , ADAS cells altered the growth inhibitory environment of the lesioned cord and contributed to axon migration despite their inability to undergo neural differentiation . Based on these results , further research is warranted into the mechanisms by which ADAS cells create a growth permissive environment in the lesioned spinal cord .
URI: http : / /hdl .handle .net /2152 .5 /302
Date: 2007-08-08

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Adipose-Derived Stromal Cells Contribute To Spinal Cord Repair But Are Not Neural-Crest Derived Stem Cells. Graduate School of Biomedical Sciences. Available electronically from http : / /hdl .handle .net /2152 .5 /302 .

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