Elucidating the Role of Yeast Lipin (PAH1) in Lipid Droplet Biogenesis

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Title: Elucidating the Role of Yeast Lipin (PAH1) in Lipid Droplet Biogenesis
Author: Adeyo, Oludotun
Abstract: Lipid droplets are unique organelles important for a host of cellular functions including the storage of neutral lipids , but factors that regulate the biogenesis and maintenance of these organelles remain relatively unknown . The primary focus of this dissertation will be to understand the role of the phosphatidic acid hydrolase (Pah1p ) in the biogenesis of lipid droplets in Saccharomyces cerevisiae . Pah1p is an enzyme that converts phosphatidic acid to diacylglycerol , and its absence or elimination of its catalytic activity results in the accumulation of neutral lipids within membranes of the endoplasmic reticulum . Furthermore , lipid droplet formation is facilitated by diacylglycerol through a mechanism that appears to be independent of diacylglycrol’s role as a substrate for triglyceride biosynthesis . Finally , lipid droplets originated from regions of the endoplasmic reticulum where the Pah1p activators were located . The second part of this dissertation will focus on the lipodystrophy related protein Fld1p and its association with lipid droplets . Droplets always associate with Fld1p , and in the absence of lipid droplets , Fld1p is localized as patches distributed throughout the endoplasmic reticulum . In addition , induced lipid droplets originate from these Fld1 patches . I conclude from this work that diacylglycerol facilitates lipid droplet formation and that Fld1p is somehow involved in the biogenesis or maintenance of these organelles . [Keywords : Lipid droplet , Diacylglycerol , lipin , steryl esters , endoplasmic reticulum , Triacylglycerol , phosphatidic acid]
URI: http : / /hdl .handle .net /2152 .5 /1012
Date: 2012-07-17


Elucidating the Role of Yeast Lipin (PAH1) in Lipid Droplet Biogenesis. Graduate School of Biomedical Sciences. Available electronically from http : / /hdl .handle .net /2152 .5 /1012 .

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