| dc.contributor |
D . Mark Estes |
|
| dc.date |
2011 -12 -20T16 :05 :46Z |
|
| dc.date |
2010 -09 -28 |
|
| dc.date |
2011 -12 -20T16 :05 :46Z |
|
| dc.date |
2008 -12 -31 |
|
| dc.date |
2008 -09 -18 |
|
| dc.date.accessioned |
2012 -03 -01T21 :54 :40Z |
|
| dc.date.available |
2012 -03 -01T21 :54 :40Z |
|
| dc.date.issued |
2012 -03 -01 |
|
| dc.identifier |
etd -12312008 -061540 |
|
| dc.identifier |
http : / /hdl .handle .net /2152 .3 /300 |
|
| dc.identifier.uri |
http : / /hdl .handle .net /2152 .3 /300 |
|
| dc.description |
The development of improved vaccines against tuberculosis (TB ) is directly linked to the investigation of new and better correlates of protection after vaccination against TB . Cloning and characterization of bovine homologues of the antimicrobial protein granulysin (Bo -lysin ) and perforin by our group could be used as potential biomarkers for TB vaccination efficacy . In the present study we examined the kinetics of granulysin , perforin , IFNgamma and Fas -L responses to Mycobacterium bovis purified protein derivative (PPD ) stimulation by peripheral blood mononuclear cells from M . bovis DeltaRD1 - , BCG - and non -vaccinated cattle . Gene expression profiles following PPD stimulation showed significant increases in transcripts for granulysin and IFNgamma in both CD4+ and CD8+ T cells in BCG -vaccinated as compared to non -vaccinated animals . Perforin and IFNgamma examined by flow cytometry , showed a difference of 1 -2 % more PPD -specific cells in BCG -vaccinated than non -vaccinated animals . In the vaccine trial , granulysin and perforin were significantly increased in both vaccine groups as compared to control after vaccination and challenge . IFNgamma expression was increased only after vaccination and secretion was higher in the control , as compared to both vaccine groups demonstrating no correlation with protection upon vaccination . In summary , results shown here provide evidence that granulysin and perforin are prospective candidates as biomarkers of protection after vaccination against TB . |
|
| dc.format |
electronic |
|
| dc.language |
eng |
|
| dc.rights |
Copyright © is held by the author . Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works . |
|
| dc.subject |
vaccination |
|
| dc.subject |
perforin |
|
| dc.subject |
Mycobacterium bovis |
|
| dc.subject |
granulysin |
|
| dc.subject |
biomarkers |
|
| dc.title |
Evaluation of granulysin and perforin as candidate biomarkers for protection following vaccination with Mycobacterium bovis BCG or M . bovis DeltaRD1 |
|
| dc.type |
text |
|
| dc.type |
dissertation |
|