Evaluation of granulysin and perforin as candidate biomarkers for protection following vaccination with Mycobacterium bovis BCG or M. bovis DeltaRD1

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dc.contributor.advisor D . Mark Estes en_US
dc.contributor.committeeMember Slobodan Paessler en_US
dc.contributor.committeeMember Janice J . Endsley en_US
dc.contributor.committeeMember Gregg N . Milligan en_US
dc.contributor.committeeMember Bernardo Villarreal -Ramos en_US
dc.contributor.committeeMember Alfredo G . Torres en_US
dc.creator Charles Fernando Capinos Scherer en_US
dc.date.accessioned 2011 -12 -20T16 :05 :46Z
dc.date.accessioned 2014 -02 -19T22 :05 :50Z
dc.date.available 2010 -09 -28 en_US
dc.date.available 2011 -12 -20T16 :05 :46Z
dc.date.available 2014 -02 -19T22 :05 :50Z
dc.date.created 2008 -12 -31 en_US
dc.date.issued 2008 -09 -18 en_US
dc.identifier.other etd -12312008 -061540 en_US
dc.identifier.uri http : / /hdl .handle .net /2152 .3 /300
dc.description.abstract The development of improved vaccines against tuberculosis (TB ) is directly linked to the investigation of new and better correlates of protection after vaccination against TB . Cloning and characterization of bovine homologues of the antimicrobial protein granulysin (Bo -lysin ) and perforin by our group could be used as potential biomarkers for TB vaccination efficacy . In the present study we examined the kinetics of granulysin , perforin , IFNgamma and Fas -L responses to Mycobacterium bovis purified protein derivative (PPD ) stimulation by peripheral blood mononuclear cells from M . bovis DeltaRD1 - , BCG - and non -vaccinated cattle . Gene expression profiles following PPD stimulation showed significant increases in transcripts for granulysin and IFNgamma in both CD4+ and CD8+ T cells in BCG -vaccinated as compared to non -vaccinated animals . Perforin and IFNgamma examined by flow cytometry , showed a difference of 1 -2 % more PPD -specific cells in BCG -vaccinated than non -vaccinated animals . In the vaccine trial , granulysin and perforin were significantly increased in both vaccine groups as compared to control after vaccination and challenge . IFNgamma expression was increased only after vaccination and secretion was higher in the control , as compared to both vaccine groups demonstrating no correlation with protection upon vaccination . In summary , results shown here provide evidence that granulysin and perforin are prospective candidates as biomarkers of protection after vaccination against TB . en_US
dc.format.medium electronic en_US
dc.language.iso eng en_US
dc.rights Copyright © is held by the author . Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works . en_US
dc.subject vaccination en_US
dc.subject perforin en_US
dc.subject Mycobacterium bovis en_US
dc.subject granulysin en_US
dc.subject biomarkers en_US
dc.title Evaluation of granulysin and perforin as candidate biomarkers for protection following vaccination with Mycobacterium bovis BCG or M . bovis DeltaRD1 en_US
dc.type.genre dissertation en_US
dc.type.material text en_US
thesis.degree.name PhD en_US
thesis.degree.level Doctoral en_US
thesis.degree.grantor The University of Texas Medical Branch en_US
thesis.degree.department Microbiology and Immunology en_US

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Evaluation of granulysin and perforin as candidate biomarkers for protection following vaccination with Mycobacterium bovis BCG or M. bovis DeltaRD1. Doctoral dissertation, The University of Texas Medical Branch. Available electronically from http : / /hdl .handle .net /2152 .3 /300 .

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