Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis

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Title: Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis
Author: Byun, Bohyun
Abstract: Light is a very important factor affecting every aspect of plant development . Plant developmental responses to light are sensitive to the direction , intensity , color , and duration of light . Light is perceived by an extensive set of photoreceptors that includes the red /far -red light ?absorbing phytochromes and blue /UV -A light ?absorbing cryptochromes . The Arabidopsis mutant seedling hyper -responsive to light 6 (shl6 ) has exaggerated developmental responses to available light . In the low light , shl6 seedlings have a phenotype similar to wild -type plants grown in high light , with short hypocotyls , expanded cotyledons , and well -developed first true leaves . In addition , the roots of shl6 are short and highly branched . The SHL6 gene was mapped to a position on chromosome 5 between simple sequence length polymorphism (SSLP ) markers nga249 and nga151 . Two cosmid clones from this interval (introduced by Agrobacterium -mediated transformation ) complemented the shl6 mutant phenotype . One candidate gene identified by complementation is a member of the glycosyltransferase family . The sequence of shl6 mutant differs from wild type Columbia allele of this gene (At5g13000 ) by a single nucleotide substitution in the first exon . This putative SHL6 gene encodes a member of a glycan synthase -like (GSL12 ) gene family that includes callose synthase . The ? -1 ,3 -D -glucan callose is found in the cell plate of dividing cells , in pollen mother cell walls , and pollen tubes . Callose synthase and related genes have not been previously implicated in developmental responses to light . We also observed that 90 % of Col -0 anthers showed high callose deposition , but shl6 mutant did not display callose deposition in the anthers . The pollen viability in the shl6 was lower than Col -0 . The epidermal cell elongation in shl6 hypocotyls was reduced when compared with Col -0 . Therefore , we conclude that the mutation in light -regulated SHL6 /GSL12 was involved in the synthesis of callose as well as light signaling .
URI: http : / /hdl .handle .net /1969 .1 /ETD -TAMU -2818
Date: 2009-05-15

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Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis. Available electronically from http : / /hdl .handle .net /1969 .1 /ETD -TAMU -2818 .

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