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Description:
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Contagious Bovine Pleuropneumonia (CBPP ) , caused by Mycoplasma mycoides
mycoides small colony (MmmSC ) , is a devastating respiratory disease of cattle in Africa ,
Asia and the Middle East . Little investigation has been done on molecular disease
pathogenesis and host response beyond soluble cytokine detection . This study developed
and characterized models for three strains of MmmSC of varying severity . Strains used
were Gladysdale , Ondangwa and Shawawa . Samples of bronchoalveolar lavage fluid ,
bronchial biopsy , nasal epithelial cells and blood were obtained prior to and at weekly
time points post -infection . Microarray analysis of RNA extracted from samples revealed
host cellular pathways and genes important in the pathogenesis of CBPP , including
multiple immune system and inflammatory response pathways . A number of pathways
whose influence on disease pathogenesis was not immediately clear were also activated ,
including pathways involved in amino acid synthesis , fat metabolism , and endocrine
hormone responses . Microarray results were confirmed with real -time polymerase chain
reaction (RT -PCR ) of selected genes . Comparative RT -PCR analysis of selected genes between the three strains of MmmSC revealed genes possibly responsible for differential
strain virulence , including interleukins 1B , 6 , 8 , and 18 and the gene nuclear factor of
kappa light polypeptide gene enhancer in B cells inhibitor , alpha (NFKBIA ) . A similar
analysis of selected genes between survivors and nonsurvivors of the virulent Gladysdale
strain of MmmSC suggested genes involved in survival , including interleukin 8 ,
calmodulin 2 (CALM2 ) , and NFKBIA . Avenues of additional study were identified . |