In vivo infection biology of contagious bovine pleuropneumonia

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Title: In vivo infection biology of contagious bovine pleuropneumonia
Author: Gull, Tamara Brownsey
Abstract: Contagious Bovine Pleuropneumonia (CBPP ) , caused by Mycoplasma mycoides mycoides small colony (MmmSC ) , is a devastating respiratory disease of cattle in Africa , Asia and the Middle East . Little investigation has been done on molecular disease pathogenesis and host response beyond soluble cytokine detection . This study developed and characterized models for three strains of MmmSC of varying severity . Strains used were Gladysdale , Ondangwa and Shawawa . Samples of bronchoalveolar lavage fluid , bronchial biopsy , nasal epithelial cells and blood were obtained prior to and at weekly time points post -infection . Microarray analysis of RNA extracted from samples revealed host cellular pathways and genes important in the pathogenesis of CBPP , including multiple immune system and inflammatory response pathways . A number of pathways whose influence on disease pathogenesis was not immediately clear were also activated , including pathways involved in amino acid synthesis , fat metabolism , and endocrine hormone responses . Microarray results were confirmed with real -time polymerase chain reaction (RT -PCR ) of selected genes . Comparative RT -PCR analysis of selected genes between the three strains of MmmSC revealed genes possibly responsible for differential strain virulence , including interleukins 1B , 6 , 8 , and 18 and the gene nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor , alpha (NFKBIA ) . A similar analysis of selected genes between survivors and nonsurvivors of the virulent Gladysdale strain of MmmSC suggested genes involved in survival , including interleukin 8 , calmodulin 2 (CALM2 ) , and NFKBIA . Avenues of additional study were identified .
URI: http : / /hdl .handle .net /1969 .1 /ETD -TAMU -2611
Date: 2009-05-15


In vivo infection biology of contagious bovine pleuropneumonia. Available electronically from http : / /hdl .handle .net /1969 .1 /ETD -TAMU -2611 .

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