Effect of n-3 PUFAs on markers of inflammation in arthritic horses

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Title: Effect of n-3 PUFAs on markers of inflammation in arthritic horses
Author: Manhart, Denise Rae
Abstract: Sixteen horses with at least one arthritic joint were randomly divided into two groups . The control group (n=8 ) was fed a control ration at 1 % BW in grain . The treatment group (n=8 ) was fed an isocaloric diet similar to the control diet with additional n -3 polyunsaturated fatty acids (PUFAs ) in the form of two pelleted supplements . Coastal hay was fed free choice , and both groups consumed their respective diet for 90 days . On d 0 , 30 , 60 , and 90 synovial fluid was collected from one arthritic joint on each horse , and blood samples were collected every 15 days . Synovial fluid was analyzed for Tumor Necrosis Factor - ? , Interleukin -1 , and white blood cell concentration , and plasma was analyzed for fibrinogen and Prostaglandin E2 . Force plate analysis was used to determine changes in weight distribution throughout the trial . Fatty acid analysis revealed the main n3 supplied by the supplements was docosahexaenoic acid (C22 :6n3 ) . Treatment horses consumed 9 .3 g docosahexaenoic acid daily , while control horses consumed only 0 .42 g daily . A reduction in concentrate intake also allowed treatment horses to consume 25 .45 g less of linoleic acid (C18 :2n6 )per day . Excluding hay , the n6 :n3 ratio of the treatment diet was 5 :1 compared to the control diet with a ratio of 11 :1 . Analysis of plasma fatty acid profiles revealed treatment horses experienced an increase in plasma docosahexaenoic acid , along with a decrease in linoleic acid (C18 :2n6 ) . Total plasma n6 :n3 ratio of treatment horses was 23 :1 , as opposed to 27 :1 in the control horses . Treatment horses had significantly lower synovial fluid white blood cell concentration and plasma Prostaglandin E2 (P < 0 .05 ) . A trend towards decreased fibrinogen (P = 0 .076 ) was also seen in the treatment horses . Synovial fluid TNF - ? and IL -1 concentrations were not obtained due to problems with the assay kits or procedures . Force plate data from seven horses was analyzed . No significant increase in weight placed on arthritic limbs (P = 0 .12 ) was seen . This data provides further evidence that a decrease in the n6 :n3 ratio of the diet and plasma can lead to a decrease in the production of inflammatory compounds in arthritic joints .
URI: http : / /hdl .handle .net /1969 .1 /ETD -TAMU -1413
Date: 2009-05-15


Effect of n-3 PUFAs on markers of inflammation in arthritic horses. Available electronically from http : / /hdl .handle .net /1969 .1 /ETD -TAMU -1413 .

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