Uncovering the circadian output pathways of Neurospora crassa

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Title: Uncovering the circadian output pathways of Neurospora crassa
Author: Vitalini, Michael William
Abstract: The ubiquity of circadian systems has allowed their characterization in a broad range of model systems , which has greatly improved knowledge of how these systems are organized and the vast range of cellular and organismal processes under circadian control . Most of the advances , however , have come in describing the central oscillators of these systems , and , in some cases , the input pathways used to coordinate these oscillators to external time . Very little progress has been made in understanding the output pathways that allow circadian systems to regulate the breadth of processes shown to be clock -controlled . A genetic selection was designed to obtain mutations in genes involved in circadianregulated expression of the Neurospora crassa ccg -1 and ccg -2 genes . Some , but not all , of the strains obtained display altered regulation of more than one ccg as well as an ?Easlike ? appearance on solid media , and altered circadian period on race tubes . The data suggest a model in which output from the clock to these two genes is through a single , bifurcated pathway . The cloning of the gene mutated (rrg -1 ) in one of the strains from the above selection led to the first molecular description of a circadian output pathway in Neurospora , the HOG MAP kinase pathway . The HOG pathway has been previously described with regard to its role in the osmotic -stress response . The discovery of the involvement of rrg -1 in circadian regulation of ccg -1 and ccg -2 led to the discovery of regulation of the HOG pathway by the circadian clock . The data indicate that osmotic stress information and time -of -day information are transduced through the HOG pathway and implicate a role for the clock in preparing the organism for daily occurrences of hyperosmotic stress associated with sun exposure . The genetic selection , and the description of the HOG pathway with regard to circadian output , provide a basis for further characterization of circadian output in Neurospora . The ubiquity of MAP kinase pathways , such as the HOG pathway , and the observed similarities in the mechanisms of circadian clock function across multiple phyla , indicate that these findings may well be applicable to other model systems .
URI: http : / /hdl .handle .net /1969 .1 /ETD -TAMU -1338
Date: 2009-05-15

Citation

Uncovering the circadian output pathways of Neurospora crassa. Available electronically from http : / /hdl .handle .net /1969 .1 /ETD -TAMU -1338 .

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