Identification and confirmation of molecular markers and orange flesh color associated with major QTL for high beta-carotene content in muskmelon

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Title: Identification and confirmation of molecular markers and orange flesh color associated with major QTL for high beta-carotene content in muskmelon
Author: Napier, Alexandra Bamberger
Abstract: Beta -carotene presence or absence in muskmelon is controlled by two genes , green flesh gf and white flesh wf . In its dominant form the wf gene is responsible for orange flesh color ; however , the epistatic interactions of gf and wf can create three flesh colors : orange , white and green . Two F2 populations , consisting of 77 greenhouse grown and 117 field grown plants , from the cross of ?Sunrise ? (white fleshed ) by ?TAM Uvalde ? (orange fleshed ) , were used to examine the relationships of beta -carotene content , flesh color , and flesh color intensity . Bulk segregent analysis was used with RAPD markers to identify molecular markers associated with high beta -carotene content . Flesh color and flesh color intensity both had significant relationships with beta -carotene content . A significant correlation between total soluble solids and beta -carotene content was also found . Molecular markers were identified in both F2 populations and all significant , associated markers from ?TAM Uvalde ? were linked with WF . A single QTL was also found to be linked with the WF locus . The identified QTL can be used to screen potential breeding lines for high beta -carotene . It was also confirmed that the visual ratings of flesh color intensity can be reliably used to select high beta -carotene content melons .
URI: http : / /hdl .handle .net /1969 .1 /ETD -TAMU -1136
Date: 2009-05-15

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Identification and confirmation of molecular markers and orange flesh color associated with major QTL for high beta-carotene content in muskmelon. Available electronically from http : / /hdl .handle .net /1969 .1 /ETD -TAMU -1136 .

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