Collection, focusing, and metering of DNA in microchannels using addressable electrode arrays for portable low-power bioanalysis

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Title: Collection, focusing, and metering of DNA in microchannels using addressable electrode arrays for portable low-power bioanalysis
Author: Shaikh, Faisal
Abstract: Although advances in microfluidic technology have enabled increasingly sophisticated biosensing and bioassay operations to be performed at the microscale , many of these applications employ such small amounts of charged biomolecules (DNA , proteins , peptides ) that they must first be pre -concentrated to a detectable level . Efficient strategies for precisely handling minute quantities of biomolecules in microchannel geometries are critically needed , however it has proven challenging to achieve simultaneous concentration , focusing , and metering capabilities with currentgeneration sample injection technology . Using microfluidic chips incorporating arrays of individually addressable microfabricated electrodes , we demonstrate that DNA can be sequentially concentrated , focused into a narrow zone , metered , and injected into an analysis channel . The technique used in this research transports charged biomolecules between active electrodes upon application of a small potential difference (1 V ) , and is capable of achieving orders of magnitude concentration increases within a small device footprint . The collected samples are highly focused , with sample zone size and shape defined solely by electrode geometry . In addition to achieving the objectives of the research project , this setup was found to provide added functionality as a label -free biomolecule detection technique due to the formation of light scattering phases of charged biomolecules on top of the capture electrode .
URI: http : / /hdl .handle .net /1969 .1 /86005
Date: 2008-10-10

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Collection, focusing, and metering of DNA in microchannels using addressable electrode arrays for portable low-power bioanalysis. Available electronically from http : / /hdl .handle .net /1969 .1 /86005 .

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