Developing luminescent nanoprobes for labeling focal adhesion complex proteins and performing combined AFM-TIRF imaging of these conjugates

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Title: Developing luminescent nanoprobes for labeling focal adhesion complex proteins and performing combined AFM-TIRF imaging of these conjugates
Author: Nathwani, Bhavik Bharat
Abstract: Recent progress in the field of semiconductor nanocrystals or Quantum Dots (QDs ) has seen them find wider acceptance as a tool in biomedical research labs . As produced , high quality QDs synthesized by high temperature organometallic synthesis , are coated with a hydrophobic ligand . Therefore , they must be further processed to be soluble in water and made biocompatible . A process to coat the QDs with silk fibroin , a fibrous protein derived from the Bombyx mori silk worm , is described . Following the coating process , the characterization of size , optical properties and biocompatibility profile of these particle systems is described . In addition , conjugation of the silk fibroin coated QDs to different labeling proteins such as phalloidin and streptavidin is described . Proteins on the surface of ovarian cancer cells (HeyA8 ) and of cytoskeletal components participating in the formation of focal adhesion complex (FAC ) , such as F -actin in endothelial cells (HUVECS ) were labeled using the bio -conjugated QDs . Various imaging techniques such as epi -fluorescence , TIRF and AFM were used to study the QD labeled cells . Overall the project has produced luminescent nanoprobes that enable the study of FAC formation dynamics and potentially a better in vivo fluorescent marker tool .
URI: http : / /hdl .handle .net /1969 .1 /85904
Date: 2008-10-10

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Developing luminescent nanoprobes for labeling focal adhesion complex proteins and performing combined AFM-TIRF imaging of these conjugates. Available electronically from http : / /hdl .handle .net /1969 .1 /85904 .

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