The characterization, functional expression, and localization of the first arthropod myokinin receptor from the southern cattle tick, Boophilus microplus (Acari: ixodidae)

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Title: The characterization, functional expression, and localization of the first arthropod myokinin receptor from the southern cattle tick, Boophilus microplus (Acari: ixodidae)
Author: Holmes, Steven P.
Abstract: Myokinins are invertebrate neuropeptides with myotropic and diuretic activity . The lymnokinin receptor from the snail Lymnaea stagnalis was the only previously identified myokinin receptor . A cDNA encoding a neuropeptide receptor was cloned from the southern cattle tick , Boophilus microplus . The deduced amino acid sequence was 40 % identical to the lymnokinin receptor . The receptor transcript is present in all tick life stages as determined by semiquantitative RT -PCR . When expressed in mammalian CHO -K1 cells , myokinins at nanomolar concentrations induced increases in intracellular calcium as measured by fluorescent cytometry . The rank order of potency for peptides tested was FFFSWS -NH2 ?FFFSWG -NH2 ?FFSWG -NH2 >FYSWG -NH2 >muscakinin >lymnokinin > >APTGFFGVR -NH2 . The receptor coupled to a pertussis toxin insensitive G protein . Absence of extracellular calcium did not inhibit the calcium response , indicating the release of Ca2+ from intracellular stores . Receptor transcript was detected by RT -PCR in the dissected synganglia , ovaries , salivary glands , guts and Malpighian tubules of partially engorged adult female ticks . It is concluded that the B . microplus receptor is the first myokinin receptor cloned from an arthropod , and the first neuropeptide receptor known from the Acari . The presence of this receptor transcript in multiple tissues and all life stages suggests a multifunctional role in ticks .
URI: http : / /hdl .handle .net /1969 .1 /60
Date: 2004-09-30

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The characterization, functional expression, and localization of the first arthropod myokinin receptor from the southern cattle tick, Boophilus microplus (Acari: ixodidae). Available electronically from http : / /hdl .handle .net /1969 .1 /60 .

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