Detection of aldehydes in lung cancer cell culture by gas chromatography/mass spectrometry and solid-phase microextraction with on-fiber derivatization

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Title: Detection of aldehydes in lung cancer cell culture by gas chromatography/mass spectrometry and solid-phase microextraction with on-fiber derivatization
Author: Shan, Guangqing
Abstract: Aldehydes in lung cancer cell culture have been investigated using gas chromatography /mass spectrometry and solid -phase microextraction with on -fiber derivatization . In this study , the poly (dimethylsiloxane /divinylbenzene (PDMS /DVB ) fiber was used and o -2 ,3 ,4 ,5 ,6 - (pentafluorobenzyl ) hydroxylamine hydrochloride (PFBHA ) was first loaded on the fiber . Aldehydes in the headspace of lung cancer cell culture were extracted by solid -phase microextraction (SPME ) fiber and subsequently derivatized by PFBHA on the fiber . Finally , the aldehyde oximes formed on the fiber were analyzed by gas chromatography /mass spectrometry (GC /MS ) . Using this method , acetaldehyde decrease was found in both non -small lung cancer cell cultures studied compared to the medium control study . The results of spiking the cell culture with acetaldehyde solution showed that 5 million SK -MES -1 cell lines could consume up to 4 .5 uM acetaldehyde in 15 -ml medium , and 5 million NCI -H522 cell lines could consume 5 .9 uM acetaldehyde in 15 -ml medium . The decrease of acetaldehyde may contribute to the metabolism of lung cancer cells . It was proved that GC /MS and SPME with on -fiber derivatization is a simple , rapid , sensitive and solvent -free method for the detection of aldehydes in lung cancer cell culture .
URI: http : / /hdl .handle .net /1969 .1 /5891
Date: 2007-09-17

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Detection of aldehydes in lung cancer cell culture by gas chromatography/mass spectrometry and solid-phase microextraction with on-fiber derivatization. Available electronically from http : / /hdl .handle .net /1969 .1 /5891 .

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