Enhancement of a fluorescent sensor for monitoring glucose concentration in diabetic patients

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Title: Enhancement of a fluorescent sensor for monitoring glucose concentration in diabetic patients
Author: Ibey, Bennett Luke
Abstract: The need for overnight and continuous monitoring of glucose levels in diabetic patients is profound , especially among juveniles . Implantation of a chemical assay which responds optically to changes in glucose concentration shows promise as a technology capable of continuously monitoring blood sugar with little invasion into the body . Previous fluorescent chemical assays , based on the affinity binding reaction between Concanavalin A protein and dextran , performed well but suffered from limited dermal penetration . In this work , a novel replacement for the dextran molecule (glycosylated dendrimer ) was fabricated and tested to determine if it would improve the overall response of the sensing chemistry to glucose . Experiments were carried out and it was found that the assay ? ? ? ? ? ?s functionality was based on the controlled aggregation of the Con A protein and the modified dendrimer molecule . This new assay proved to be specific to glucose , reversible , and independent of fluorophore dye attached to the protein . This research was furthered by encapsulation of the new assay into a PEG hydrogel which showed response to glucose but , due to leeching , did not perform well under repeated exposures . A new method for encapsulation was proposed based on poration of the hydrogel to create micropores capable of holding the assay chemistry and allowing it to react to incoming glucose , while the surrounding polymer restricted leeching . Preliminary results with previous assays proved the potential of a mannitol based poration procedure , but unforeseen complications in lyophilization of the new sensor assay restricted its completion . Due to instability of Con A in solution , it was hypothesized that the immobilization of it onto the surface of an active substrate would increase its stability overtime as seen in previous works . The immobilization procedure was performed on Con A for both polystyrene spheres and gold (nanoshells and colloid ) . Both results showed an adequate amount of protein on the surface of the particles , but little binding activity was demonstrated . Overall , the improvements to the sensor chemistry response were notable and the potential for stabilization and enhancement of the response through the use of an active substrate is promising .
URI: http : / /hdl .handle .net /1969 .1 /4991
Date: 2007-04-25

Citation

Enhancement of a fluorescent sensor for monitoring glucose concentration in diabetic patients. Available electronically from http : / /hdl .handle .net /1969 .1 /4991 .

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