Characterization and applications of microfluidic devices based on immobilized biomaterials

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Title: Characterization and applications of microfluidic devices based on immobilized biomaterials
Author: Heo, Jinseok
Abstract: Microfluidic biosensors and bioreactors based on immobilized biomaterials are described in this dissertation . Photocrosslinkable hydrogel or polymeric microbeads were used as a supporting matrix for immobilizing E .coli or enzymes in a microfluidic device . This dissertation covers a microfluidic bioreactor based on hydrogel -entrapped E .coli , a microfluidic biosensor based on an array of hydrogel -entrapped enzymes , and a microfluidic bioreactor based on microbead -immobilized enzymes . Hydrogel micropatches containing E .coli were fabricated within a microfluidic channel by in -situ photopolymerization . The cells were viable in the hydrogel micropatch and their membranes could be porated by lysating agents . Entrapment of viable cells within hydrogels , followed by lysis , could provide a convenient means for preparing biocatalysts without the need for enzyme extraction and purification . Our results suggested that hydrogel -entrapped cells , immobilized within microfluidic channels , can act as sensors for small molecules and as bioreactors for carrying out reactions . A microfluidic biosensor based on an array of hydrogel -entrapped enzymes could be used to simultaneously detect different concentrations of the same analyte or multiple analyte in real time . The concentration of an enzyme inhibitor could be quantified using the same basic approach . Isolations of the microchannels within different microfluidic channels could eliminate the possibility of cross talk between enzymes . Finally , we characterized microfluidic bioreactors packed with microbead -immobilized enzymes that can carry out sequential , two -step enzyme -catalyzed reactions under flow conditions . The overall efficiency of the reactors depended on the spatial relationship of the two enzymes immobilized on the beads . Digital simulations confirmed the experimental results .
URI: http : / /hdl .handle .net /1969 .1 /4688
Date: 2007-04-25

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Characterization and applications of microfluidic devices based on immobilized biomaterials. Available electronically from http : / /hdl .handle .net /1969 .1 /4688 .

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