Topics in living cell miultiphoton laser scanning microscopy (MPLSM) image analysis

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Title: Topics in living cell miultiphoton laser scanning microscopy (MPLSM) image analysis
Author: Zhang, Weimin
Abstract: Multiphoton laser scanning microscopy (MPLSM ) is an advanced fluorescence imaging technology which can produce a less noisy microscope image and minimize the damage in living tissue . The MPLSM image in this research is the dehydroergosterol (DHE , a fluorescent sterol which closely mimics those of cholesterol in lipoproteins and membranes ) on living cell's plasma membrane area . The objective is to use a statistical image analysis method to describe how cholesterol is distributed on a living cell's membrane . Statistical image analysis methods applied in this research include image segmentation /classification and spatial analysis . In image segmentation analysis , we design a supervised learning method by using smoothing technique with rank statistics . This approach is especially useful in a situation where we have only very limited information of classes we want to segment . We also apply unsupervised leaning methods on the image data . In image data spatial analysis , we explore the spatial correlation of segmented data by a Monte Carlo test . Our research shows that the distributions of DHE exhibit a spatially aggregated pattern . We fit two aggregated point pattern models , an area -interaction process model and a Poisson cluster process model , to the data . For the area interaction process model , we design algorithms for maximum pseudo -likelihood estimator and Monte Carlo maximum likelihood estimator under lattice data setting . For the Poisson Cluster process parameter estimation , the method for implicit statistical model parameter estimate is used . A group of simulation studies shows that the Monte Carlo maximum estimation method produces consistent parameter estimates . The goodness -of -fit tests show that we cannot reject both models . We propose to use the area interaction process model in further research .
URI: http : / /hdl .handle .net /1969 .1 /4412
Date: 2006-10-30

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Topics in living cell miultiphoton laser scanning microscopy (MPLSM) image analysis. Available electronically from http : / /hdl .handle .net /1969 .1 /4412 .

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