Neurogenesis in the dentate gyrus of age-matched calcium ion channel mutant mice, leaner and tottering

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Title: Neurogenesis in the dentate gyrus of age-matched calcium ion channel mutant mice, leaner and tottering
Author: Wills, Sarah Ellen
Abstract: Homozygous leaner and tottering mice have a mutation in the alpha -1A subunit , which cause a decrease in calcium ion current through these channels leading to altered calcium homeostasis . These channels are highly expressed in several areas of the brain including the hippocampus . Neurogenesis in adults happens only in two specific areas : the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles . Since P /Q -type channels are highly expressed in the hippocampus , we expect altered calcium homeostasis might affect neural proliferation in the mutant mice . The aims of this thesis were : (1 ) to determine if there was a decrease in neural proliferation in the dentate gyrus of adult leaner and tottering mice when compared to age -matched wild -type mice and (2 ) to determine if the new cells are neurons or glial cells and if there is a difference in proportion when compared to wild -type mice . We used bromodeoxyuridine (BrdU ) to label the new cells . We examined proliferation rates in 100 -150 day old mice and in 42 -50 day old mice . We doublelabeled some newly formed cells using two different fluorescent tags to determine if the cells were neurons or glial cells . These studies showed that cell proliferation is indeed decreased in leaner and tottering mice at three months of age when compared to the wild -type mice . However , when we looked at mice that were 42 -50 days old , we found a significant increase in cell proliferation in leaner and tottering mice compared to agematched control mice . When we looked at the proportion of double -labeled cells (neurons versus glia ) , there was no difference among genotypes for either age group . For both age -groups , there were about 90 % new neurons and 20 % new glial cells for the P100 -150 mice and 85 % new neurons and 10 % new glial cells for the P42 -50 mice . Since we do not see a change in proportion of double -labeled cells at either age group , it is probable that differentiation patterns are not being affected . Based on these observations , we predict that the altered calcium homeostasis that is probably occurring in the mutant mice is affecting cell proliferation , but not differentiation .
URI: http : / /hdl .handle .net /1969 .1 /4410
Date: 2006-10-30

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Neurogenesis in the dentate gyrus of age-matched calcium ion channel mutant mice, leaner and tottering. Available electronically from http : / /hdl .handle .net /1969 .1 /4410 .

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