Glucose oxidation in heart-type fatty acid binding protein null mice

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Title: Glucose oxidation in heart-type fatty acid binding protein null mice
Author: Adhikari, Sean
Abstract: Heart -type fatty acid binding protein (H -FABP ) is a major fatty acid binding factor in skeletal muscles . Genetic lack of H -FABP severely impairs the esterification and oxidation of exogenous fatty acids in soleus muscles isolated from chow -fed mice (CHOW -solei ) and high fat diet -fed mice (HFD -solei ) , and prevents the HFD -induced accumulation of muscle triglycerides . Here , we examined the impact of H -FABP deficiency on the relationship between fatty acid utilization and glucose oxidation . Glucose oxidation was measured in isolated soleus muscles in the presence or absence of 1 mM palmitate (simple protocol ) or in the absence of fatty acid after preincubation with 1 mM palmitate (complex protocol ) . With the simple protocol , the mutation slightly reduced glucose oxidation in CHOW -muscles , but markedly increased it in HFDmuscles ; unexpectedly , this pattern was not altered by the addition of palmitate , which reduced glucose oxidation in both CHOW - and HFD -solei irrespective of the mutation . In the complex protocol , the mutation first inhibited the synthesis and accumulation of triglycerides and then their mobilization ; with this protocol , the mutation increased glucose oxidation in both CHOW - and HFD -solei . We conclude : (i ) H -FABP mediates a non -acute inhibition of muscle glucose oxidation by fatty acids , likely by enabling both the accumulation and mobilidoes not mediate the acute inhibitory effect of extracellular fatty acids on muscle glucose oxidation ; (iii ) H -FABP affects muscle glucose oxidation in opposing ways , with inhibition prevailing at high muscle triglyceride contents .zation of a critical mass of muscle triglycerides ; (ii ) H -FABP
URI: http : / /hdl .handle .net /1969 .1 /4391
Date: 2006-10-30

Citation

Glucose oxidation in heart-type fatty acid binding protein null mice. Available electronically from http : / /hdl .handle .net /1969 .1 /4391 .

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