Babesia microti cysteine protease-1 as a target for vaccine development

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Title: Babesia microti cysteine protease-1 as a target for vaccine development
Author: James, Allison Melissa
Abstract: Babesia species have a worldwide distribution , affecting a wide range of mammalian hosts . The major route of transmission is inoculation by an infected Ixodid tick . Babesia species of major economic concern are those that cause bovine and equine babesiosis . Historically , bovine Babesia species , Babesia bovis and Babesia bigemina caused significant economic losses in the United States in the 1860 ? ? ? ? ? ?s , as thousands of cattle died . Also , outbreaks of equine babesiosis , caused by Babesia equi or Babesia caballi , have occurred in the United States resulting in the death of some horses and millions of dollars in losses . A constant risk of reinfection with bovine and equine Babesia species exists , as stray and smuggled animals from Mexico , where bovine babesiosis is endemic , may carry infected ticks as they cross the border , and , thousands of horses from B . equiand B . caballi -endemic regions are imported through Florida every year . Vaccines have been developed for a number of Babesia species , none of which result in sterile immunity . The live attenuated vaccine is the most commonly used vaccine against Babesia species . However , the basis for the vaccine is to maintain a carrier state in order to prevent disease . Other vaccine designs have been developed to invoke protection without a carrier state but have been unsuccessful . It has been shown that the cysteine protease is important in the life cycle of a number of parasitic organisms , making it a good target for vaccine development . The vaccine design for this study incorporated the cysteine protease of Babesia microti . Babesia microti naturally infects Peromyscus leucopus (white -footed mouse ) and is the major cause of human babesiosis in the United States . Using B . microti in the vaccine design allowed for the use of a mouse model to determine whether the cysteine protease of other economically important Babesia species may make a good vaccine target . The vaccine design incorporated a prime -boost strategy , priming with DNA encoding the cysteine protease and boosting two times with either DNA encoding the cysteine protease or cysteine protease peptide , followed by parasite challenge . Analysis of daily percent parasitemias , packed cell volume , and seroconversion of all groups revealed that a protective immune response against B . microti was not elicited by this vaccine strategy .
URI: http : / /hdl .handle .net /1969 .1 /4192
Date: 2006-10-30

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Babesia microti cysteine protease-1 as a target for vaccine development. Available electronically from http : / /hdl .handle .net /1969 .1 /4192 .

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