Design, testing and optimization of a microfluidic device for capture and concentration of bacteria

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Title: Design, testing and optimization of a microfluidic device for capture and concentration of bacteria
Author: Cherla, Srinivas
Abstract: Effective detection of bacterial pathogens in large sample volumes is a challenging problem . Pre -concentration routines currently in practice before the actual detection process are cumbersome and hard to automate . An effort is made to address the problem of volume discrepancy between day -to -day samples and the concentrated samples needed for analysis . Principles of conceptual design are used in formulating the ? ? ? ? ? ?Need Statement ? ? ? ? ? ? , ? ? ? ? ? ?Function Structure ? ? ? ? ? ? and in identifying the ? ? ? ? ? ?Critical Design Parameters ? ? ? ? ? ? and ? ? ? ? ? ?Design Constraints ? ? ? ? ? ? . Electrokinetic phenomena are used to exploit the surface charges on bacteria . Electrophoresis is used to transport the bacteria to electrode surface and ? ? ? ? ? ?Electrostatic trapping ? ? ? ? ? ? is then used to capture these microbes on the electrode surface . The captured microbes can then be concentrated in a concentrator unit . A prototype microfluidic device is fabricated for showing the proof of concept . Optimization is done to minimize hydraulic power consumption and wetted volume . Observations from the initial prototype device along with the optimization results are used in building a new prototype device . Operation of this device is demonstrated by capture of bacteria from flow . Qualitative studies are conducted and preliminary quantification is also done .
URI: http : / /hdl .handle .net /1969 .1 /4140
Date: 2006-10-30

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Design, testing and optimization of a microfluidic device for capture and concentration of bacteria. Available electronically from http : / /hdl .handle .net /1969 .1 /4140 .

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