Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays

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Title: Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays
Author: Zhang, Jinglan
Abstract: The 12 -oxo -phytodienoic acid reductases (OPRs ) are enzymes of the octadecanoid pathway which converts linolenic acid to a phytohormone , jasmonic acid . Bioinformatics analysis of ESTs and genomic sequences from available private and public databases revealed that the maize genome encodes eight different OPR genes . This number of maize OPR genes has been independently confirmed by Southern blot analysis and by mapping of individual OPR genes to maize chromosomes using oat maize chromosome addition lines . Survey of massively parallel signature sequencing (MPSS ) assays revealed that transcripts of each OPR gene accumulate differentially in diverse organs of maize plants . This data suggested that individual OPR genes may have a distinct function in development . Similarly , RNA blot analysis revealed that distinct OPR genes are differentially regulated in response to stress hormones , wounding or pathogen infection . ZmOPR1 and ZmOPR2 appear to have important functions in defense responses to pathogens because they are transiently induced by salicylic acid (SA ) , chitooligosaccharides and by infection with Cochliobolus carbonum , Bipolaris maydis and Fusarium verticillioides and not by wounding . In contrast to these two genes , ZmOPR6 and ZmOPR7 /8 are highly induced by wounding and treatments with wound -associated signaling molecules jasmonic acid , ethylene and abscisic acid . ZmOPR6 and ZmOPR7 /8 are not induced by SA treatments or pathogen infections suggesting their specific involvement in wound -induced defense responses . Possible functions of specific OPR genes are discussed .
URI: http : / /hdl .handle .net /1969 .1 /3332
Date: 2006-04-12


Genomic analysis of 12-oxo-phytodienoic acid reductase genes of Zea mays. Available electronically from http : / /hdl .handle .net /1969 .1 /3332 .

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