Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts

Show full item record

Title: Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts
Author: Waters, Paulette Francesca
Abstract: Molecular and in vitro comparisons were performed using two isolates of Encephalitozoon hellem , one from an avian host and one from a human host , and one isolate of Encephalitozoon cuniculi from a rabbit . The molecular comparisons were performed by amplifying and sequencing the gene coding for a zinc metallo -aminopeptidase from cDNA and gDNA obtained from each of the isolates . The E . hellem sequences shared >99 % identity between each other and 70 % identity with the E . cuniculi sequences . Conserved HEXXH and GXMEN motifs located within the sequences classify the protein as an aminopeptidase of the M1 family , with at least one zinc atom required for catalytic activity . In vitro growth comparisons of the isolates described above were performed under simulated "mammalian and avian conditions" . The models utilized mammalian and avian cell lines and sera at incubation temperatures of 37 ?C and 40 ?C , respectively . Three separate experiments were performed . E . cuniculi grew best under the mammalian model and significantly better than both E . hellem isolates under this model . The E . hellem isolates were able to infect and replicate under both the mammalian and avian models , which reflects the zoonotic potential of these isolates .
URI: http : / /hdl .handle .net /1969 .1 /330
Date: 2004-09-30

Citation

Molecular and in vitro growth comparisons of Encephalitozoon hellem isolates from human and bird hosts. Available electronically from http : / /hdl .handle .net /1969 .1 /330 .

Files in this item

Files Size Format View
etd-tamu-2003A-2003032812-Wate-1.pdf 1.268Mb application/pdf View/Open

This item appears in the following Collection(s)

Show full item record

Search DSpace

Advanced Search

Browse