Cancer diagnostics using dynamic near-infrared optical imaging and fluorescent contrast agents

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Title: Cancer diagnostics using dynamic near-infrared optical imaging and fluorescent contrast agents
Author: Gurfinkel, Mikhail
Abstract: A new optical imaging modality has been developed for small animal in vivo imaging of near -infrared fluorescence resulting from fluorescent contrast agents specifically targeted to molecular markers of cancer . The imaging system is comprised of an intensified charge -coupled device (ICCD ) for the detection of ultra -low levels of re -emitted fluorescence following the delivery of an expanded beam of excitation light . The design of the ICCD detection system allows for both continuous wave (CW ) and frequency -domain modes of operation . Since the accurate acquisition of frequency -domain photon migration (FDPM ) data is important for tomographic imaging , the imaging system was also validated using experimentally obtained FDPM measurements of homogenous turbid media and diffusion theory to obtain estimates of the optical properties characteristic of the media . The experiments demonstrated that the absorption and reduced scattering coefficients are determined least accurately when relative rel measurements of average light intensity IDC are employed either alone or in a rel combination with relative modulation amplitude data IAC and /or relative phase shift data rel . However , when FDPM measurements of are employed either alone or in rel combination with IAC data , the absorption and reduced scattering coefficients may be found accurate to within 15 % and 11 % , respectively , of the values obtained from standard single -pixel measurements ; a result that suggests that FDPM data obtained from an ICCD detection system may in fact be useful in tomographic imaging . Furthermore , intensified -detection allows for sub -second exposure times , permitting the acquisition of dynamic fluorescence images immediately following administration of the contrast agent . Experimental results demonstrate that when coupled with a suitable pharmacokinetic model describing targeted dye distribution throughout the body , dynamic fluorescence imaging may be used to discriminate spontaneous canine adenocarcinoma from normal mammary tissue . A separate experiment demonstrates that pharmacokinetic analysis of dynamic fluorescence images enables one to estimate the rate constant governing Kaposi's sarcoma tumor uptake of an integrin -targeted dye and integrin receptor turnover rate . The rate constant for uptake was calculated to be 0 .16 -sec -1 while the turnover rate of the integrin receptor was estimated to occur within 24 -hours .
URI: http : / /hdl .handle .net /1969 .1 /3162
Date: 2006-04-12

Citation

Cancer diagnostics using dynamic near-infrared optical imaging and fluorescent contrast agents. Available electronically from http : / /hdl .handle .net /1969 .1 /3162 .

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