Effects of cytosine-phosphate-guanosine oligodinucleotides (CpG-ODNs) on oral immunization with protein antigen or replicating parasite

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Title: Effects of cytosine-phosphate-guanosine oligodinucleotides (CpG-ODNs) on oral immunization with protein antigen or replicating parasite
Author: Ameiss, Keith Allen
Abstract: The purpose of this research was to investigate selected methods of mucosal immunization for commercial chickens . Induction of mucosal immunity in commercial chickens through the use of orally administered subunit vaccines or through immunomodulation of the host ? ?s response to live vaccines may be a viable means to control enteric infections in commercial poultry . In the present investigations we evaluated a means for delivering protein antigen in the drinking water and the use of CpG -ODNs , a recently reported mucosal adjuvant , in order to both improve this response and to modulate the host ? ?s immune response when vaccinated with field strains of Eimeria acervulina and Eimeria tenella . In order to evaluate the efficacy of immunizing commercial poultry with subunit vaccines through the drinking water we chose the model antigen Bovine Serum Albumin (BSA ) . Chicks were administered BSA via intraperitoneal (I .P . ) injection , oral crop gavage , or orally through the addition of BSA to the drinking water . These experiments demonstrated the efficacy of drinking water administration to induce antibodyproduction in the serum , intestine , and bile . When BSA was co -administered with CpGODNs we observed a modest increase in this response dependent upon dose . To evaluate the immunomodulation of the host response to live parasite using CpG -ODNs we used three administration models . The first was a single dose of CpGODNs with a trickle immunization regime of Eimeria acervulina . The second was coadministration of CpG -ODNs with a clinical dose of Eimeria acervulina or tenella . The third was pre -administration of CpG -ODNs 24 hours prior to the clinical dose of either species . These studies demonstrate that the first and third models were effective in reducing lesions and improving performance .
URI: http : / /hdl .handle .net /1969 .1 /2421
Date: 2005-08-29

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Effects of cytosine-phosphate-guanosine oligodinucleotides (CpG-ODNs) on oral immunization with protein antigen or replicating parasite. Available electronically from http : / /hdl .handle .net /1969 .1 /2421 .

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