Chemopreventive effects of curcumin and green tea on B[a]P-induced carcinogenesis in the hamster cheek pouch

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Title: Chemopreventive effects of curcumin and green tea on B[a]P-induced carcinogenesis in the hamster cheek pouch
Author: Brandon, Jimi Lynn
Abstract: The present study was carried out to examine the chemopreventive effects of curcumin and green tea polyphenols on the hamster cheek pouch carcinogenesis model . This model of oral carcinogenesis has been widely used in chemoprevention studies , however , these studies have been limited to the use of DMBA as the carcinogenic agent . We have developed a protocol of carcinogenesis in the hamster cheek pouch using B[a]P , a broadly distributed environmental carcinogen , formed as a by -product of the combustion of organic materials including cigarette smoke . B[a]P - induced tumors in the hamster cheek pouch are primarily endophytic squamous cell carcinomas that closely resemble squamous cell carcinomas of the human oral mucosa . The cheek pouch of male Syrian hamsters were treated topically for eight weeks with 0 .6 % curcumin , 6 .0 % curcumin , 2 .5 % green tea polyphenols , or 5 .0 % green tea polyphenols , 3 times per week 30 minutes prior to the application of 2 .0 % B[a]P . The animals were sacrificed 24 hours and 72 hours after the last treatments . Short -term mechanistic markers of malignant progression were used to determine effects of each compound . Cellular proliferation , assessed by bromodeoxyuridine (Brdu ) incorporation , p53 protein accumulation , and apoptotic activity were evaluated . The results of the present study demonstrated that 0 .6 % curcumin and 2 .5 % green tea polyphenols had strong inhibitory effects on cellular proliferation and p53 protein accumulation . And 6 .0 % curcumin and 5 .0 % green tea polyphenols appeared to induce apoptosis . Our data suggest that curcumin and green tea polyphenols may have a plausible chemopreventive effect on oral carcinogenesis in the hamster cheek pouch model .
URI: http : / /hdl .handle .net /1969 .1 /2410
Date: 2005-08-29

Citation

Chemopreventive effects of curcumin and green tea on B[a]P-induced carcinogenesis in the hamster cheek pouch. Available electronically from http : / /hdl .handle .net /1969 .1 /2410 .

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