Photo-initiated cross-linked polyacrylamide gels for microdevice electrophoresis

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Title: Photo-initiated cross-linked polyacrylamide gels for microdevice electrophoresis
Author: Agrawal, Shilpa
Abstract: Photo -polymerized cross -linked polyacrylamide gels are becoming increasingly important for use in micro -fabricated DNA electrophoresis systems because they allow a concentrated sieving matrix to be precisely positioned at any location within a complex micro -channel network . The rate of photo -initiation in the free radical gel polymerization reaction , however , can exert a strong influence on the resulting gel structure . Experimental data on separation resolution of single stranded DNA (ssDNA ) in photoinitiated polyacrylamide gels is very sparse . In this study , we investigate the performance of ssDNA electrophoresis in an ALF Express automated DNA sequencer using various photo -initiation chemistries . Cross -linked polyacrylamide gels with concentrations ranging from 6 to 12 %T were prepared using riboflavin , methylene blue , irgacure 651 and ReproGel (AP Biotech ) photo -initiators . Separation resolution is compared with that attained in cross -linked polyacrylamide gels prepared using conventional chemical initiators (e .g . ammonium persulfate / Tetramethylethylenediamine ) in order to determine the polymerization conditions necessary for optimum performance .
URI: http : / /hdl .handle .net /1969 .1 /2311
Date: 2005-08-29

Citation

Photo-initiated cross-linked polyacrylamide gels for microdevice electrophoresis. Available electronically from http : / /hdl .handle .net /1969 .1 /2311 .

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