Effects of Cytosine-phosphate-Guanosine Oligodeoxynucleotides (CpG-ODN) on vaccination and immunization of neonatal chickens

Show full item record

Title: Effects of Cytosine-phosphate-Guanosine Oligodeoxynucleotides (CpG-ODN) on vaccination and immunization of neonatal chickens
Author: Barri, Adriana
Abstract: The objective of this investigation was to evaluate the effects of administering CpG -ODN to commercial strain chickens as a potential adjuvant to vaccination against Salmonella , Eimeria spp . , and Newcastle disease virus , or immunization to bovine serum albumin (BSA ) . During Experiment 1 , which evaluated the dual application of CpG -ODN and a Newcastle disease virus vaccine , in the first of three replicate trials , on day 28 of the experiment , animals in the Vaccine + CpG 1 & 14 experimental group were observed to have the highest levels of (p <0 .05 ) anti -NDV IgG in serum . These levels were elevated above levels in animals from all other experimental groups . This suggestion for an adjuvant effect associated with CpG -ODN administration was not supported in the remaining two trials of experiment 1 . Experiment 2 evaluated the potential for CpG -ODN to adjuvant a commercial live oocyst coccidial vaccine when applied by an oral route to neonatal broiler chickens . Overall , when body weight gain during challenge , development of intestinal lesions , and anti -Eimeria IgG levels were evaluated , vaccine administration alone was demonstrated to provide the best measure of protection among animals in all experimental groups , including those receiving either CpG -ODN or Non CpG -ODN . Experiment 3 investigated the simultaneous administration of CpG -ODN or Non -CpG ODN and a commercially acquired Salmonella typhimurium vaccine to SCWL chickens . Similar to experiments 1 and 2 , antigen specific IgG responses in serum and indices of protection against field strain Salmonella challenge were variable and inconsistent . Anti -BSA IgG levels were compared in broiler and SCWL chickens immunized against BSA by a drinking water route of administration alone , or in combination with two different concentrations of CpG -ODN or Non CpG -ODN in experiment 4 . The only observation where CpG -ODN and BSA co -administration resulted in anti -BSA IgG levels that were elevated above BSA alone immunized chickens was measured in broilers at day 19 post -final immunization . Taken together , given the variable results reported in this investigation related to the co -administration of ODN and vaccine or protein antigen , these data are largely inconclusive for suggesting that CpG -ODN can effectively adjuvant humoral immune responses in commercial strain chickens .
URI: http : / /hdl .handle .net /1969 .1 /1550
Date: 2005-02-17

Citation

Effects of Cytosine-phosphate-Guanosine Oligodeoxynucleotides (CpG-ODN) on vaccination and immunization of neonatal chickens. Available electronically from http : / /hdl .handle .net /1969 .1 /1550 .

Files in this item

Files Size Format View
etd-tamu-2004C-POSC-Barri.pdf 352.7Kb application/pdf View/Open

This item appears in the following Collection(s)

Show full item record

Search DSpace

Advanced Search

Browse