Characterization of the 3' terminal 42 nucleotide host protein binding element of the mouse hepatitis virus 3' untranslated region

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Title: Characterization of the 3' terminal 42 nucleotide host protein binding element of the mouse hepatitis virus 3' untranslated region
Author: Johnson, Reed Findley
Abstract: Mouse Hepatitis virus (MHV ) is a member of the coronavirus family in the order Nidovirales . The 32 kb genome contains cis -acting sequences necessary for replication of the viral genome . Those cis -acting sequences have been shown to bind host proteins , and binding of those proteins is necessary for virus replication . One of the cis -acting elements is the 3' terminal 42 nucleotide host protein binding element . Previous work has demonstrated that mitochondrial aconitase , mitochondrial heat shock protein 70 , heat shock protein 60 and heat shock protein 40 bind to the 3' terminal 42 nucleotide host protein binding element . We demonstrated that RNA secondary structure of the 3' terminal 42 nucleotide host protein binding element is necessary for host protein binding in vitro . We also demonstrate that primary structure of the 3' terminal 42 nucleotide host protein binding element is necessary for viral replication by targeted recombination . DI replication assays infer that the 3' terminal 42 nucleotide host protein binding element plays a role in positive strand synthesis from the negative strand template . Current studies involve the infectious cDNA clone , which will provide definitive answers on the role of the 3' terminal 42 nucleotide host protein binding element in MHV replication .
URI: http : / /hdl .handle .net /1969 .1 /125
Date: 2004-09-30

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Characterization of the 3' terminal 42 nucleotide host protein binding element of the mouse hepatitis virus 3' untranslated region. Available electronically from http : / /hdl .handle .net /1969 .1 /125 .

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